TIE1 Human

TIE1 Human Recombinant
Cat. No.
BT14934
Source

HEK293 Cells.

Synonyms

Tyrosine-protein kinase receptor Tie-1, TIE1, TIE, JTK14, LMPHM11.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TIE1 Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 977 amino acids (22-759 a.a) and having a molecular mass of 106.8kDa.
TIE1 is fused to a 239 amino acid hIgG-His-Tag at C-terminus and is purified by proprietary chromatographic techniques.

Product Specs

Introduction
TIE1, an angiopoitein receptor, plays a crucial role in hematopoiesis, angiogenesis, and vasculogenesis. Primarily expressed on endothelial and hematopoietic progenitor cells, TIE1 is found in immature hematopoietic cells and platelets. It upregulates cell adhesion molecules (CAMs) such as VCAM-1, ICAM-1, and E-selectin through a p38-dependent mechanism. TIE1 exhibits proinflammatory effects and contributes to endothelial inflammatory diseases like atherosclerosis.
Description
Recombinant Human TIE1 protein, produced in HEK293 cells, is a single, glycosylated polypeptide chain. It consists of 977 amino acids (22-759 a.a), resulting in a molecular mass of 106.8 kDa. The protein includes a C-terminal fusion of a 239 amino acid hIgG-His-Tag. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The TIE1 solution (0.5 mg/ml) is formulated in 20mM Tris-HCl (pH 8.0), 0.1M NaCl, and 50% glycerol.
Stability
For short-term storage (2-4 weeks), refrigerate at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms

Tyrosine-protein kinase receptor Tie-1, TIE1, TIE, JTK14, LMPHM11.

Source

HEK293 Cells.

Amino Acid Sequence

AVDLTLLANL RLTDPQRFFL TCVSGEAGAG RGSDAWGPPL LLEKDDRIVR TPPGPPLRLA RNGSHQVTLR GFSKPSDLVG VFSCVGGAGA RRTRVIYVHN SPGAHLLPDK VTHTVNKGDT AVLSARVHKE KQTDVIWKSN GSYFYTLDWH EAQDGRFLLQ LPNVQPPSSG IYSATYLEAS PLGSAFFRLI VRGCGAGRWG PGCTKECPGC LHGGVCHDHD GECVCPPGFT GTRCEQACRE GRFGQSCQEQ CPGISGCRGL TFCLPDPYGC SCGSGWRGSQ CQEACAPGHF GADCRLQCQC QNGGTCDRFS GCVCPSGWHG VHCEKSDRIP QILNMASELE FNLETMPRIN CAAAGNPFPV RGSIELRKPD GTVLLSTKAI VEPEKTTAEF EVPRLVLADS GFWECRVSTS GGQDSRRFKV NVKVPPVPLA APRLLTKQSR QLVVSPLVSF SGDGPISTVR LHYRPQDSTM DWSTIVVDPS ENVTLMNLRP KTGYSVRVQL SRPGEGGEGA WGPPTLMTTD CPEPLLQPWL EGWHVEGTDR LRVSWSLPLV PGPLVGDGFL LRLWDGTRGQ ERRENVSSPQ ARTALLTGLT PGTHYQLDVQ LYHCTLLGPA SPPAHVLLPP SGPPAPRHLH AQALSDSEIQ LTWKHPEALP GPISKYVVEV QVAGGAGDPL WIDVDRPEET STIIRGLNAS TRYLFRMRAS IQGLGDWSNT VEESTLGNGL QAEGPVQESR AAEEGLDQLE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH

Product Science Overview

Introduction

TIE1, also known as Tyrosine-protein kinase receptor Tie-1, is a receptor tyrosine kinase that plays a crucial role in the vascular system. It is predominantly expressed in endothelial cells and is essential for normal embryonic vascular development . TIE1 is part of a receptor tyrosine kinase subfamily that includes TIE2, and both receptors share unique structural characteristics, including immunoglobulin-like domains, epidermal growth factor (EGF)-like domains, and fibronectin type III-like repeats .

Structure and Function

TIE1 is characterized by its complex structure, which includes:

  • Two immunoglobulin-like domains flanking three EGF-like domains.
  • Three fibronectin type III-like repeats in the extracellular region.
  • A split tyrosine kinase domain in the cytoplasmic region .

This structural configuration allows TIE1 to participate in various signaling pathways that regulate endothelial cell survival, migration, and differentiation. Although TIE1 is an orphan receptor (meaning its ligand is not well-defined), it is known to interact with other molecules in the angiopoietin signaling pathway, contributing to vascular stability and integrity .

Recombinant TIE1

Recombinant TIE1 (Human) is a laboratory-produced version of the TIE1 protein, designed for research purposes. It is typically expressed in systems such as HEK 293 cells or mouse myeloma cell lines (NS0-derived), ensuring high purity and biological activity . The recombinant protein is often tagged with a His tag for easy purification and is available in various formulations, including carrier-free versions to avoid interference in specific assays .

Applications

Recombinant TIE1 is used in a variety of research applications, including:

  • Cell signaling studies: To understand the role of TIE1 in endothelial cell function and vascular development.
  • Drug development: As a target for developing therapies aimed at vascular diseases and cancer.
  • Biochemical assays: For studying protein-protein interactions and receptor-ligand binding .

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