TICAM2 Human

Toll-Like Receptor Adaptor Molecule 2 Human Recombinant

Cat. No.

BT24467

Source

E.coli.

Synonyms

Toll-like receptor adaptor molecule 2, TIRP, TRAM, TICAM-2, Putative NF-kappa-B-activating protein 502, Toll-like receptor adaptor protein 3, TIRAP3, cytoplasmic adaptor, TIR domain-containing adapter molecule 2, toll/interleukin-1 receptor (TIR) domain-containing adapter protein, TRIF-related adaptor molecule.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 85% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

TICAM2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 259 amino acids (1-235) and having a molecular mass of 29.4kDa.
TICAM2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

TICAM2, a member of the Toll/interleukin-1 receptor (TIR) family, plays a crucial role in mediating immune responses. This cytoplasmic protein interacts with TLR4 and TICAM-1, facilitating the transmission of LPS-TLR4 signals to TICAM-1. This signaling cascade subsequently activates IRF-3. Additionally, TICAM2 participates in IL1-induced NF-kappa-B activation, functioning upstream of key signaling molecules such as IRAK1, IRAK2, TRAF6, and IKBKB.

Description

Recombinant human TICAM2, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 259 amino acids (residues 1-235). The protein exhibits a molecular weight of 29.4 kDa. For purification and detection purposes, a 24 amino acid His-tag is fused to the N-terminus. Purification is achieved through proprietary chromatographic techniques.

Physical Appearance

Clear, colorless solution that has been sterilized by filtration.

Formulation

The TICAM2 solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, and 10% glycerol.

Stability

For short-term storage (up to 2-4 weeks), the TICAM2 solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage to maintain protein stability. Repeated freezing and thawing should be avoided.

Purity

The purity of the TICAM2 protein is greater than 85%, as determined by SDS-PAGE analysis.

Synonyms

Source

E.coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMGIGKS KINSCPLSLS WGKRHSVDTS PGYHESDSKK SEDLSLCNVA EHSNTTEGPT GKQEGAQSVE EMFEEEAEEE VFLKFVILHA EDDTDEALRV QNLLQDDFGI KPGIIFAEMP CGRQHLQNLD DAVNGSAWTI LLLTENFLRD TWCNFQFYTS LMNSVNRQHK YNSVIPMRPL NNPLPRERTP FALQTINALE EESRGFPTQV ERIFQESVYK TQQTIWKETR NMVQRQFIA

Product Science Overview

Introduction

Toll-Like Receptor Adaptor Molecule 2 (TRAM), also known as TICAM2, is a crucial component in the Toll-like receptor (TLR) signaling pathway. This pathway plays a significant role in the innate immune response, which is the body’s first line of defense against pathogens. TRAM is a cytoplasmic protein that is involved in the signaling processes of TLRs, particularly TLR4.

Structure and Function

TRAM is a member of the Toll/interleukin-1 receptor (TIR) family, which includes various proteins involved in immune response signaling. The TIR domain is essential for the protein-protein interactions that facilitate signal transduction. TRAM specifically interacts with TLR4 and another adaptor protein, TICAM-1 (also known as TRIF), to mediate downstream signaling events.

Role in TLR Signaling

TLR4 is known for recognizing lipopolysaccharides (LPS) from Gram-negative bacteria. Upon LPS recognition, TLR4 undergoes dimerization and recruits adaptor proteins, including TRAM. TRAM then facilitates the transfer of the signal to TRIF, which subsequently activates transcription factors such as IRF3 and NF-κB. These transcription factors lead to the production of type I interferons and other pro-inflammatory cytokines.

Biological Significance

The TLR signaling pathway, including the role of TRAM, is vital for initiating an effective immune response. Dysregulation of this pathway can lead to various inflammatory diseases and conditions. For instance, TLR2 and TLR4 signaling through TRAM and TRIF has been implicated in the pathogenesis of atherosclerosis, where it mediates inflammation and matrix degradation .

Research and Therapeutic Potential

Given its central role in immune signaling, TRAM is a potential target for therapeutic interventions aimed at modulating immune responses. Inhibitors or modulators of TRAM could be developed to treat conditions characterized by excessive inflammation, such as autoimmune diseases and chronic inflammatory conditions.

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TICAM2 Human

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