TIAL1 Human

TIAL1 Human Recombinant
Cat. No.
BT24408
Source
Escherichia Coli.
Synonyms
TCBP, TIAR, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TIA-1-related protein.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TIAL1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 398 amino acids (1-375 a.a) and having a molecular mass of 44.0kDa.TIAL1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
TIAL1, a member of the RNA-binding protein family, possesses three RNA recognition motifs (RRMs) and exhibits binding affinity for adenine and uridine-rich elements within mRNA and pre-mRNA molecules of a wide array of genes. This protein plays a crucial role in regulating various cellular processes, including translational control, splicing, and apoptosis. Notably, different isoforms of TIAL1 exhibit distinct functionalities with respect to post-transcriptional silencing.
Description
Recombinant human TIAL1, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 398 amino acids (residues 1-375). With a molecular weight of 44.0 kDa, TIAL1 is fused to a 24-amino acid His-tag at its N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
TIAL1 protein solution at a concentration of 0.5 mg/ml. The solution is buffered with phosphate-buffered saline (pH 7.4) and supplemented with 50% glycerol, 2 mM DTT, and 1 mM EDTA.
Stability
For short-term storage (2-4 weeks), maintain the product at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity exceeds 90.0% as assessed by SDS-PAGE analysis.
Synonyms
TCBP, TIAR, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TIA-1-related protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMMEDDGQ PRTLYVGNLS RDVTEVLILQ LFSQIGPCKS CKMITEHTSN DPYCFVEFYE HRDAAAALAA MNGRKILGKE VKVNWATTPS SQKKDTSNHF HVFVGDLSPE ITTEDIKSAF APFGKISDAR VVKDMATGKS KGYGFVSFYN KLDAENAIVH MGGQWLGGRQ IRTNWATRKP PAPKSTQENN TKQLRFEDVV NQSSPKNCTV YCGGIASGLT DQLMRQTFSP FGQIMEIRVF PEKGYSFVRF STHESAAHAI VSVNGTTIEG HVVKCYWGKE SPDMTKNFQQ VDYSQWGQWS QVYGNPQQYG QYMANGWQVP PYGVYGQPWN QQGFGVDQSP SAAWMGGFGA QPPQGQAPPP VIPPPNQAGY GMASYQTQ.

Product Science Overview

Structure and Function

TIAL1 contains three RNA recognition motifs (RRMs) that enable it to bind to adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes . This binding capability allows TIAL1 to regulate the stability and translation of these mRNAs, thereby influencing gene expression at the post-transcriptional level.

The protein is involved in several key cellular activities:

  • Translational Control: TIAL1 can repress or activate the translation of specific mRNAs, thereby controlling the production of proteins in the cell.
  • Splicing: It plays a role in the splicing of pre-mRNAs, a process essential for the generation of mature mRNAs that can be translated into proteins.
  • Apoptosis: TIAL1 is implicated in the regulation of programmed cell death, a vital process for maintaining cellular homeostasis and eliminating damaged or harmful cells .
Recombinant TIAL1

Recombinant TIAL1 is a form of the protein that is produced through recombinant DNA technology. This involves inserting the TIAL1 gene into a suitable expression system, such as Escherichia coli (E. coli), to produce the protein in large quantities . The recombinant protein typically includes a His-tag at the N-terminus to facilitate purification and detection.

The recombinant TIAL1 protein is used in various research applications, including:

  • Biochemical Studies: To investigate the protein’s structure, function, and interactions with other molecules.
  • Cellular Assays: To study the effects of TIAL1 on cellular processes such as translation, splicing, and apoptosis.
  • Drug Development: To screen for compounds that can modulate the activity of TIAL1, which may have therapeutic potential for diseases involving dysregulated RNA processing .
Storage and Stability

Recombinant TIAL1 is typically formulated in a buffer containing phosphate-buffered saline (PBS), glycerol, dithiothreitol (DTT), and ethylenediaminetetraacetic acid (EDTA) to maintain its stability . It should be stored at 4°C for short-term use and at -20°C for long-term storage. Avoiding freeze-thaw cycles is essential to preserve the protein’s integrity .

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