THRSP Human

Thyroid Hormone Responsive Human Recombinant
Cat. No.
BT24170
Source
Escherichia Coli.
Synonyms
Lpgp, LPGP1, S14, SPOT14, THRP, Spot 14 protein.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

THRSP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 169 amino acids (1-146 a.a.) and having a molecular mass of 18.9kDa.
THRSP is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Thyroid Hormone Responsive (THRSP), a member of the SPOT14 family, shares similarities with the S14 gene product. The expression of S14, a rat gene, is confined to the liver and adipose tissue and is regulated by nutritional and hormonal factors. THRSP expression is observed in the liver, adipocytes, particularly in lipomatous modules, and lipogenic breast cancers, suggesting its potential role in regulating tumor lipid metabolism.
Description
Recombinant THRSP Human, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 169 amino acids (1-146 a.a.). With a molecular mass of 18.9 kDa, it comprises the THRSP protein fused to a 23 amino acid His-tag at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear solution, sterile filtered.
Formulation
THRSP protein solution (1 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90.0% as determined by SDS-PAGE analysis.
Synonyms
Lpgp, LPGP1, S14, SPOT14, THRP, Spot 14 protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMQVLTKR YPKNCLLTVM DRYAAEVHNM EQVVMIPSLL RDVQLSGPGG QAQAEAPDLY TYFTMLKAIC VDVDHGLLPR EEWQAKVAGS EENGTAETEE VEDESASGEL DLEAQFHLHF SSLHHILMHL TEKAQEVTRK YQEMTGQVW.

Product Science Overview

Structure and Function

TSH is a glycoprotein hormone composed of two subunits: alpha (α) and beta (β). The alpha subunit is common to other glycoprotein hormones like luteinizing hormone (LH) and follicle-stimulating hormone (FSH), while the beta subunit is unique and confers biological specificity. The recombinant form of TSH is produced by co-transfecting a human α-subunit cDNA with a human β-subunit partial genomic clone into Chinese Hamster Ovary (CHO) cells .

Production Process

The production of rhTSH involves several steps:

  1. Genetic Engineering: CHO cells are genetically engineered to express high levels of rhTSH.
  2. Culturing: These cells are cultured on microcarrier beads in serum-free conditions.
  3. Purification: The rhTSH-containing media is clarified and purified using ion exchange, dye, and gel filtration chromatographies. The final product is highly pure, with a purity level greater than 99% .
Clinical Applications

Recombinant human TSH is primarily used in the management of thyroid cancer. It stimulates the uptake of radioactive iodine (131 I) and the release of thyroglobulin (Tg) from thyroid remnants and metastases, aiding in the detection and treatment of thyroid carcinoma . Additionally, rhTSH is used in diagnostic tests to evaluate thyroid function without the need for patients to discontinue thyroid hormone therapy .

Advantages and Limitations

Advantages:

  • Non-invasive: Allows for thyroid function testing without stopping thyroid hormone therapy.
  • High Purity: The recombinant form is highly pure and biologically active.
  • Effective: Proven to be effective in stimulating thyroid tissue for diagnostic purposes .

Limitations:

  • Cost: The production process is complex and costly.
  • Limited Efficacy: Some studies indicate that rhTSH has relatively low affinity to the human TSH receptor compared to bovine or rodent TSH, which may limit its clinical efficacy .
Future Prospects

Research is ongoing to develop superactive analogues of rhTSH with increased receptor affinity, potency, and efficacy. These next-generation thyrotropins are expected to provide more effective diagnostic and therapeutic options for thyroid diseases .

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