THOC7 Human

THO Complex 7 Human Recombinant
Cat. No.
BT24066
Source
Escherichia Coli.
Synonyms
THO Complex 7, Functional Spliceosome-Associated Protein 24, NIF3L1BP1, Ngg1-Interacting Factor 3-Like Protein 1-Binding Protein 1, Ngg1 Interacting Factor 3 Like 1 Binding Protein 1, NIF3L1-Binding Protein 1, HTREX30, FSAP24, THO Complex 7 Homolog (Drosophila), THO Complex Subunit 7 Homolog, THO Complex 7 Homolog, fSAP24, hTREX30.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

THOC7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (1-204 a.a) and having a molecular mass of 26.1kDa.THOC7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
THO Complex 7, also referred to as THOC7, is part of the THO subcomplex within the larger TREX complex. This complex plays a crucial role in connecting mRNA transcription, processing, and export from the nucleus, particularly for spliced mRNA. The THOC7 gene encodes a protein essential for the export of polyadenylated RNA. Moreover, THOC7 is linked to RNA transport processes.
Description
Recombinant human THOC7, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 227 amino acids (specifically, amino acids 1 to 204). It has a molecular weight of 26.1 kDa. A 23 amino acid His-tag is fused to the N-terminus of THOC7. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The THOC7 protein solution has a concentration of 0.5 mg/ml. It is prepared in a buffer consisting of phosphate-buffered saline (pH 7.4), 50% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it should be frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity exceeds 90.0% as determined by SDS-PAGE analysis.
Synonyms
THO Complex 7, Functional Spliceosome-Associated Protein 24, NIF3L1BP1, Ngg1-Interacting Factor 3-Like Protein 1-Binding Protein 1, Ngg1 Interacting Factor 3 Like 1 Binding Protein 1, NIF3L1-Binding Protein 1, HTREX30, FSAP24, THO Complex 7 Homolog (Drosophila), THO Complex Subunit 7 Homolog, THO Complex 7 Homolog, fSAP24, hTREX30.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGAVTDD EVIRKRLLID GDGAGDDRRI NLLVKSFIKW CNSGSQEEGY SQYQRMLSTL SQCEFSMGKT LLVYDMNLRE MENYEKIYKE IECSIAGAHE KIAECKKQIL QAKRIRKNRQ EYDALAKVIQ HHPDRHETLK ELEALGKELE HLSHIKESVE DKLELRRKQF HVLLSTIHEL QQTLENDEKL SEVEEAQEAS METDPKP.

Product Science Overview

Structure and Components

The human THO complex consists of several subunits, including THOC1, THOC2, THOC3, THOC5, THOC6, and THOC7. These subunits work together to form a functional complex that interacts with other proteins involved in mRNA processing and export. The THO complex is part of the larger transcription-export (TREX) complex, which also includes UAP56/DDX39B and ALYREF .

Function in mRNA Biogenesis

The primary function of the THO complex is to facilitate the proper formation and export of mRNP particles. During transcription, the THO complex associates with nascent RNA and contributes to several steps in mRNA biogenesis, including splicing and export. The complex ensures that the mRNA is properly processed and packaged into mRNP particles that are competent for export from the nucleus .

Role in Transcription and RNA Export

The THO complex plays a critical role in the transcriptional elongation process. It helps to prevent the nascent RNA from interacting with the DNA template, thereby ensuring the efficient and accurate transcription of mRNA. Once the mRNA is transcribed, the THO complex, as part of the TREX complex, facilitates the export of the mature mRNP particles from the nucleus to the cytoplasm .

THO Complex 7 (THOC7)

THOC7 is one of the subunits of the human THO complex. It is involved in the structural integrity and function of the complex. THOC7 interacts with other subunits of the THO complex and plays a role in the multivalent interactions that are essential for the proper functioning of the TREX complex. The recombinant form of THOC7 is often used in research to study the structure and function of the THO complex .

Research and Implications

Research on the THO complex, including THOC7, has provided valuable insights into the mechanisms of mRNA biogenesis and export. Understanding the structure and function of the THO complex has implications for various biological processes, including gene expression regulation, cell differentiation, and development. Additionally, defects in the THO complex have been linked to various diseases, highlighting the importance of this complex in maintaining cellular homeostasis .

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