THBS4 Mouse
HEK293 Cells.
THBS4, Thbs4, Thbs-4, Thrombospondin-4, Thrombospondin4, thrombospondin 4, Tsp4, TSP-4, TSP, TSP4, TS.
Sterile filtered colorless solution.
Greater than 85.0% as determined by SDS-PAGE.
Description
THBS4 Mouse Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 943 amino acids (27-963a.a) and having a molecular mass of 104.3 kDa.
THBS4 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Thrombospondin-4 (THBS4), a member of the thrombospondin protein family, is an adhesive glycoprotein that plays a crucial role in cell-to-cell and cell-to-matrix interactions. As a pentameric protein, THBS4 exhibits binding affinity for heparin and calcium. Notably, it interacts with various matrix proteins, including Collagens I, II, III, V, Laminin-1, Matrilin-2, and Fibronectin. THBS4 expression is elevated in the spinal cord following peripheral nerve injury, contributing to presynaptic hypersensitivity and hyperalgesia. It is also upregulated in muscle tissue after denervation.
Recombinant THBS4 Mouse, produced in HEK293 cells, is a single, glycosylated polypeptide chain comprising 943 amino acids (27-963a.a). With a molecular weight of 104.3 kDa, it includes a 6 amino acid His tag at the C-terminus. Purification is achieved using proprietary chromatographic techniques.
A sterile, colorless solution.
The THBS4 solution (0.25mg/ml) is prepared with phosphate buffered saline (pH 7.4), 0.1mM PMSF, and 20% glycerol.
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity exceeds 85.0% as determined by SDS-PAGE analysis.
THBS4, Thbs4, Thbs-4, Thrombospondin-4, Thrombospondin4, thrombospondin 4, Tsp4, TSP-4, TSP, TSP4, TS.
HEK293 Cells.
QATPQVFDLL PSSSQRLNPS ALQPVLTDPT LHEVYLISTF KLQSKSSATI FGLYSSSDNS KYFEFTVMGR LNKAILRYLK NDGKIHLVVF NNLQLADGRR HRVLLRLSNL QRGDGSVELY LDCAQADSVR NLPRAFSGLT QNPESIELRT FQRKPQDFLE ELKLVVRGSL FQVASLQDCF LQQSEPLAAT STGDFNRQFL GQMTQLNQLL GEVKDLLRQQ VKETSFLRNT IAECQACGPL SFQSPTPNTL VPIAPPAPPT RPTRHCDSSP CFRGVRCTDT RDGFQCGPCP DGYTGNGITC SDVDECKYHP CYPGVRCVNL APGFRCDACP VGFTGPMVQG VGINFAKTNK QVCTDVDECQ NGACVLNSIC INTLGSYRCG PCKPGYTGDQ TRGCKTERSC RNPEQNPCSV HAQCIEERQG DVTCVCGVGW AGDGYVCGKD VDIDSYPDEE LPCSARNCKK DNCKYVPNSG QEDADRDGIG DACDEDADGD GILNEQDNCV LTHNIDQRNS DKDIFGDACD NCRMVLNNDQ KDTDGDGRGD ACDDDMDGDG IKNILDNCPR VPNRDQQDRD GDDVGDACDS CPDVSNPNQS DVDNDLVGDS CDTNQDSDGD GHQDSTDNCP TVINSSQLDT DKDGIGDECD DDDDNDGIPD LVPPGPDNCR LVPNPAQEDS NNDGVGDICE ADFDQDQVID HIDVCPENAE ITLTDFRAYQ TVVLDPEGDA QIDPNWVVLN QGMEIVQTMN SDPGLAVGYT AFNGVDFEGT FHVNTQTDDD YAGFIFGYQD SSSFYVVMWK QTEQTYWQAT PFRAVAEPGI QLKAVKSKTG PGEHLRNSLW HTGDTSDQVR LLWKDSRNVG WKDKVSYRWF LQHRPQVGYI RVRFYEGSEL VADSGVTIDT TMRGGRLGVF CFSQENIIWS NLKYRCNDTI PEDFQEFQTQ SFDRLDNHHH HHH
Product Science Overview
TSP-4 is involved in regulating cell-cell and cell-matrix interactions. It has been shown to support the adhesion of SVEC4-10 mouse vascular endothelial cells, with an effective dose (ED50) for this effect ranging from 0.1 to 0.5 μg/mL . This activity highlights its role in cardiovascular physiology and its potential impact on vascular health.
The recombinant mouse TSP-4 protein is produced in a carrier-free form, meaning it does not contain Bovine Serum Albumin (BSA), which can interfere with certain applications . The protein is lyophilized from a 0.2 μm filtered solution in PBS and has a purity greater than 90%, as determined by SDS-PAGE under reducing conditions .
