THAP1 Human

THAP Domain Containing, Apoptosis Associated Protein 1 Human Recombinant
Cat. No.
BT11762
Source
Escherichia Coli.
Synonyms
THAP domain-containing protein 1, THAP1, DYT6.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

THAP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 237 amino acids (1-213 a.a) and having a molecular mass of 27.5kDa.
THAP1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The THAP domain-containing protein 1 (THAP1) is a protein comprising 213 amino acids. It is exclusively found within the cell nucleus and possesses a single THAP-type zinc finger, a well-conserved DNA-binding domain. THAP1 functions as a DNA-binding transcription regulator, playing a crucial role in controlling the proliferation of endothelial cells and the progression of the G1/S cell cycle phase. Furthermore, THAP1 exhibits pro-apoptotic activity by enhancing both serum-withdrawal-induced and TNF-induced apoptosis. Notably, THAP1 protein co-localizes with the apoptosis response protein PAWR/PAR-4 in the nuclear bodies of promyelocytic leukemia (PML) cells. This co-localization suggests that THAP1 acts as a proapoptotic factor, linking PAWR to PML nuclear bodies.
Description
Recombinant human THAP1 protein, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 237 amino acids (residues 1-213). It has a molecular weight of 27.5 kDa. The THAP1 protein includes a 24 amino acid His-tag fused at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The THAP1 protein is supplied as a 1 mg/ml solution in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 2 M Urea, 10% glycerol, 1 mM DTT, and 0.2 M NaCl.
Stability
For short-term storage (up to 2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal long-term stability, adding a carrier protein (either 0.1% HSA or BSA) is advised. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the THAP1 protein is greater than 85.0% as determined by SDS-PAGE analysis.
Synonyms
THAP domain-containing protein 1, THAP1, DYT6.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMVQSCS AYGCKNRYDK DKPVSFHKFP LTRPSLCKEW EAAVRRKNFK PTKYSSICSE HFTPDCFKRE CNNKLLKENA VPTIFLCTEP HDKKEDLLEP QEQLPPPPLP PPVSQVDAAI GLLMPPLQTP VNLSVFCDHN YTVEDTMHQR KRIHQLEQQV
EKLRKKLKTA QQRCRRQERQ LEKLKEVVHF QKEKDDVSER GYVILPNDYF EIVEVPA.

Product Science Overview

Introduction

THAP Domain Containing, Apoptosis Associated Protein 1, also known as THAP1, is a protein encoded by the THAP1 gene in humans. This protein is characterized by the presence of a THAP domain, a conserved DNA-binding domain that plays a crucial role in various cellular processes, including apoptosis and transcription regulation.

Structure and Function

THAP1 contains a THAP domain, which is a zinc-dependent DNA-binding domain. This domain is involved in recognizing and binding to specific DNA sequences, thereby regulating the expression of target genes. The protein is known to colocalize with the apoptosis response protein PAWR/PAR-4 in promyelocytic leukemia (PML) nuclear bodies, functioning as a proapoptotic factor that links PAWR to PML nuclear bodies .

Role in Apoptosis

THAP1 has been shown to have pro-apoptotic activity, meaning it can promote programmed cell death. This activity is potentiated by both serum withdrawal and tumor necrosis factor (TNF)-induced apoptosis. The protein’s ability to induce apoptosis is significant in the context of cancer research, as it may provide insights into mechanisms that can be targeted for therapeutic interventions .

Clinical Significance

Mutations in the THAP1 gene have been associated with DYT6 dystonia, a hereditary movement disorder characterized by sustained, involuntary muscle contractions. This condition highlights the importance of THAP1 in maintaining normal cellular functions and its potential role in neurological disorders .

Research and Applications

Recombinant THAP1 protein is used in various research applications to study its function and interactions. Understanding the role of THAP1 in apoptosis and transcription regulation can provide valuable insights into the development of new therapeutic strategies for diseases such as cancer and dystonia .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.