TFAM Human
Transcription Factor-A Recombinant Human
Cat. No.
BT23795
Source
Escherichia Coli.
Synonyms
Transcription factor A, mitochondrial, TCF6, TCF6L2, Mitochondrial transcription factor 1, Transcription factor 6-like 2, MtTF1, mtTFA, TCF6L1, TCF6L3.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
TFAM produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (43-246.a.a) and having a molecular mass of 26.6kDa.
TFAM is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
TFAM is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
TFAM, a mitochondrial transcription factor, plays a crucial role in initiating mitochondrial transcription and participating in mitochondrial genome replication. Primarily located in the nuclei of elongated spermatids, TFAM contributes to the regulation of gene expression within the haploid male genome. Notably, TFAM has been associated with mitochondrial disorders in humans, characterized by symptoms such as ocular myopathy, exercise intolerance, and muscle wasting.
Description
Produced in E. coli, TFAM is a single, non-glycosylated polypeptide chain composed of 225 amino acids (specifically, residues 43-246). With a molecular weight of 26.6 kDa, TFAM is fused to a 21 amino acid His-tag at its N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear solution that has undergone sterile filtration.
Formulation
The TFAM protein solution, provided at a concentration of 0.25 mg/ml, is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 5 mM DTT, and 20% glycerol.
Purity
The purity of TFAM is determined to be greater than 90% using SDS-PAGE analysis.
Stability
For short-term storage (2-4 weeks), TFAM should be kept at 4°C. For extended periods, it is recommended to store the protein frozen at -20°C. Repeated freeze-thaw cycles should be avoided to maintain protein integrity.
Synonyms
Transcription factor A, mitochondrial, TCF6, TCF6L2, Mitochondrial transcription factor 1, Transcription factor 6-like 2, MtTF1, mtTFA, TCF6L1, TCF6L3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSSVLASCPK KPVSSYLRFS KEQLPIFKAQ NPDAKTTELI RRIAQRWREL PDSKKKIYQD AYRAEWQVYK EEISRFKEQL TPSQIMSLEK EIMDKHLKRK AMTKKKELTL LGKPKRPRSA YNVYVAERFQ EAKGDSPQEK LKTVKENWKN LSDSEKELYI QHAKEDETRY HNEMKSWEEQ MIEVGRKDLL RRTIKKQRKY GAEEC
Product Science Overview
Structure and Function
TF-A belongs to a family of transcription factors characterized by their ability to bind to specific DNA sequences and regulate the transcription of target genes. These proteins typically contain several functional domains, including:
- DNA-binding domain (DBD): This domain allows TF-A to bind to specific DNA sequences, usually located in the promoter regions of target genes.
- Transactivation domain: This region interacts with other proteins, such as co-activators or co-repressors, to modulate the transcriptional activity of the target gene.
- Dimerization domain: Some transcription factors, including TF-A, function as dimers, meaning they pair with another identical or similar protein to exert their regulatory effects.
Biological Properties
Recombinant human TF-A retains the essential properties of the natural protein, including its ability to bind DNA and regulate gene expression. It is produced using recombinant DNA technology, which involves inserting the gene encoding TF-A into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of the protein, which can then be purified and used for various research and therapeutic applications.
Functions and Modes of Action
TF-A plays a pivotal role in several cellular processes:
- Gene Regulation: By binding to specific DNA sequences, TF-A can either activate or repress the transcription of target genes. This regulation is crucial for maintaining cellular homeostasis and responding to external signals.
- Cell Differentiation: TF-A is involved in the differentiation of various cell types, guiding the development of specialized cells from progenitor or stem cells.
- Development: During embryonic development, TF-A helps orchestrate the expression of genes necessary for the formation of tissues and organs.
- Stress Response: TF-A can also be involved in the cellular response to stress, such as oxidative stress or DNA damage, by regulating the expression of genes involved in protective mechanisms.
Regulatory Mechanisms
The activity of TF-A is tightly regulated at multiple levels:
- Transcriptional Regulation: The expression of the TF-A gene itself can be regulated by other transcription factors, signaling pathways, and environmental cues.
- Post-translational Modifications: TF-A can undergo various modifications, such as phosphorylation, acetylation, or ubiquitination, which can alter its stability, localization, or activity.
- Protein-Protein Interactions: TF-A often interacts with other proteins, including co-activators, co-repressors, and other transcription factors, to form complexes that modulate its regulatory functions.
Applications of Recombinant Human TF-A
Recombinant human TF-A has several applications in research and medicine:
- Research Tool: It is used to study gene regulation, cellular differentiation, and developmental processes in various model systems.
- Therapeutic Potential: Recombinant TF-A can be explored as a potential therapeutic agent for diseases where gene regulation is disrupted, such as cancer or genetic disorders.
- Biotechnology: It can be used in the development of gene therapy approaches, where the controlled expression of therapeutic genes is required.
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