Tetanus antibody
Analysis by SDS-PAGE.
Description
Recombinant Anti Tetanus produced in E.Coli is a non-glycosylated, polypeptide chain containing a hexahistidine tag and having a molecular weight of 37 kDa.
Tetanus Antibody is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Tetanus is a severe infectious disease caused by the bacterium Clostridium tetani. This bacterium produces a potent neurotoxin known as tetanospasmin, which leads to muscle stiffness and spasms. Despite the availability of vaccines, tetanus remains a significant health concern, particularly in developing countries and regions affected by natural disasters or conflicts.
The tetanus toxoid is an inactivated form of the tetanus toxin used in vaccines to induce immunity against tetanus. The toxoid stimulates the immune system to produce antibodies without causing the disease. However, in some cases, there is a need for rapid and sensitive detection methods for tetanus toxin, especially in clinical diagnostics, food safety, and water monitoring.
Recombinant antibodies are engineered antibodies produced using recombinant DNA technology. They offer several advantages over traditional antibodies, including consistent production, reduced risk of contamination, and the ability to tailor their properties for specific applications. One type of recombinant antibody is the single-chain variable fragment (scFv), which consists of the variable regions of the heavy and light chains of an antibody, connected by a short linker peptide.
Phage display is a powerful technique used to produce recombinant antibodies. It involves displaying antibody fragments on the surface of bacteriophages (viruses that infect bacteria) and selecting those with high affinity for a specific antigen. This method allows for the rapid generation of high-affinity antibodies from large libraries of antibody fragments.
Researchers have utilized phage display technology to develop scFv antibodies against tetanus toxoid. The process begins with constructing a high-quality phage display antibody library. The library is then subjected to several rounds of biopanning, where phages displaying antibodies with high affinity for tetanus toxoid are selected. The selected phages are further screened using techniques such as enzyme-linked immunosorbent assay (ELISA) to identify the most promising candidates.
Once the high-affinity scFv antibodies are identified, they are expressed in bacterial systems and purified. Techniques such as sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting are used to confirm the purity and quality of the recombinant antibodies. The affinity of the scFv antibodies for tetanus toxoid is determined using ELISA, and the results indicate that these antibodies have a high affinity for the antigen.
The tetanus toxoid scFv recombinant antibodies have several potential applications:
- Clinical Diagnostics: These antibodies can be used in immunoassays to detect tetanus toxin in clinical samples, enabling early diagnosis and treatment of tetanus.
- Food Safety: The antibodies can be employed to monitor food products for contamination with tetanus toxin, ensuring food safety.
- Water Monitoring: The antibodies can be used to detect tetanus toxin in water sources, preventing the spread of the disease through contaminated water.
