TEF Human

Thyrotrophic Embryonic Factor Human Recombinant

Cat. No.

BT23553

Source

Escherichia Coli.

Synonyms

Thyrotroph embryonic factor, TEF, Thyrotroph embryonic factor isoform 1.FBG2, FBS2, FBX6, Fbx6b, F-box only protein 6, F-box protein that recognizes sugar chains 2, F-box/G-domain protein 2, FBXO6.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 85% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

TEF Human Recombinant produced in E. coli is a single polypeptide chain containing 326 amino acids (1-303) and having a molecular mass of 35.6kDa.TEF is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Thyrotroph embryonic factor (TEF) is a nuclear transcription factor belonging to the bZIP (basic region/leucine zipper) family and PAR subfamily. It binds DNA as a homodimer or heterodimer and is known to activate the TSH beta promoter. TEF levels follow a strong circadian rhythm and it can also suppress cell growth by downregulating the expression of cytokine receptor beta chains.

Description

Recombinant human TEF, produced in E. coli, is a single polypeptide chain consisting of 326 amino acids (residues 1-303) with a molecular weight of 35.6 kDa. It includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.

Physical Appearance

Clear, sterile filtered solution.

Formulation

The TEF solution (0.25 mg/ml) is buffered with 20 mM Tris-HCl (pH 8.0) and contains 10% glycerol.

Stability

For short-term storage (2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

Purity exceeding 85% as determined by SDS-PAGE analysis.

Synonyms

Thyrotroph embryonic factor, TEF, Thyrotroph embryonic factor isoform 1.FBG2, FBS2, FBX6, Fbx6b, F-box only protein 6, F-box protein that recognizes sugar chains 2, F-box/G-domain protein 2, FBXO6.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSDAGGG KKPPVDPQAG PGPGPGRAAG ERGLSGSFPL VLKKLMENPP REARLDKEKG KEKLEEDEAA AASTMAVSAS LMPPIWDKTI PYDGESFHLE YMDLDEFLLE NGIPASPTHL AHNLLLPVAE LEGKESASSS TASPPSSSTA IFQPSETVSS
TESSLEKERE TPSPIDPNCV EVDVNFNPDP ADLVLSSVPG GELFNPRKHK FAEEDLKPQP MIKKAKKVFV PDEQKDEKYW TRRKKNNVAA KRSRDARRLK ENQITIRAAF LEKENTALRT EVAELRKEVG KCKTIVSKYE TKYGPL.

Product Science Overview

Gene and Protein Structure

The TEF gene is located on chromosome 22 (22q13.2) in humans. The gene encodes a protein that is involved in DNA-binding and transcriptional regulation . The TEF protein binds to a specific DNA sequence and activates the transcription of target genes. The minimal DNA-binding sequence for TEF is 5’-[TC][AG][AG]TTA[TC][AG]-3’.

Expression and Function

TEF is expressed in a broad range of cells and tissues in adult animals . However, during embryonic development, its expression is restricted to the developing anterior pituitary gland. This coincides with the appearance of thyroid-stimulating hormone, beta (TSHB). TEF can bind to and transactivate the TSHB promoter, playing a crucial role in the regulation of thyroid hormone production .

Homology and Interactions

TEF shows homology with other members of the PAR-bZIP subfamily of transcription factors, including albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF), and chicken vitellogenin gene-binding protein (VBP). These proteins can form heterodimers and share DNA-binding and transcriptional regulatory properties .

Recombinant TEF

Recombinant TEF is produced using recombinant DNA technology, which involves inserting the TEF gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein can be used for various research and therapeutic purposes, including studying its role in thyroid hormone regulation and its potential applications in treating thyroid-related disorders.

Clinical Significance

TEF is associated with several biological processes, including the regulation of transcription by RNA polymerase II, rhythmic processes, and multicellular organism development . Dysregulation of TEF expression or function can lead to various diseases, including thyroid disorders and developmental abnormalities.

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TEF Human

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