TBPL1 Human

TBP-Like 1 Human Recombinant

Cat. No.

BT23307

Source

Escherichia Coli.

Synonyms

TATA box-binding protein-like protein 1, TBP-like protein 1, 21 kDa TBP-like protein, Second TBP of unique DNA protein, STUD, TATA box-binding protein-related factor 2, TBP-related factor 2, TBP-like factor, TBP-related protein, TBPL1, TLF, TLP, TLP21, TRF2, TRP, MGC:8389, MGC:9620.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Shipped with Ice Packs

In Stock

Quick Inquiry

Description

TBPL1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 209 amino acids (1-186 a.a) and having a molecular mass of 23.3kDa.
TBPL1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

TBP-Like 1 (TBPL1), part of the TBP family, plays a crucial role in the basal transcriptional machinery. This protein is essential for spermiogenesis and is believed to be vital for expressing developmentally regulated genes.

Description

Recombinant human TBPL1, produced in E. coli, is a non-glycosylated polypeptide chain comprising 209 amino acids (residues 1-186) with a molecular weight of 23.3 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.

Physical Appearance

Clear, colorless solution, sterilized by filtration.

Formulation

The TBPL1 protein solution is provided at a concentration of 0.25 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 50% glycerol, 2 mM DTT, and 1 mM EDTA.

Stability

For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freeze the solution at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is recommended for long-term storage. Repeated freezing and thawing should be avoided.

Purity

SDS-PAGE analysis indicates a purity greater than 90%.

Synonyms

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMDADSDV ALDILITNVV CVFRTRCHLN LRKIALEGAN VIYKRDVGKV LMKLRKPRIT ATIWSSGKII CTGATSEEEA KFGARRLARS LQKLGFQVIF TDFKVVNVLA VCNMPFEIRL PEFTKNNRPH ASYEPELHPA VCYRIKSLRA TLQIFSTGSI TVTGPNVKAV ATAVEQIYPF VFESRKEIL.

Product Science Overview

Structure and Expression

TBPL1 is a human full-length protein, typically in the 1 to 186 amino acid range. It is expressed in Escherichia coli and has a purity of over 90%, making it suitable for applications such as SDS-PAGE and mass spectrometry. The protein solution often contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol, 2mM DTT, and 1mM EDTA.

Function and Importance

TBPL1 does not bind to the TATA box, unlike the traditional TATA box-binding protein (TBP). Instead, it is involved in the transcription of genes that do not contain the TATA box. This includes the transcription of ribosomal proteins and possibly the transcription of NF1. The ability of TBPL1 to mediate transcription without binding to the TATA box highlights its unique role in gene regulation.

Applications

Recombinant TBPL1 is used in various research applications, including studies on gene expression and transcriptional regulation. Its high purity and specific expression system make it a valuable tool for scientists studying the mechanisms of transcription and the role of TBPL1 in different cellular processes .

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Product Name

Quantity&Size*

Message

TBPL1 Human

Description

Product Specs

Product Science Overview

Quick Inquiry

Category

Service