TBCA Antibody

Tubulin Folding Cofactor A, Mouse Anti Human
Cat. No.
BT1072
Source
Synonyms
Tubulin-specific chaperone A, Tubulin-folding cofactor A, CFA, TCP1-chaperonin cofactor A, TBCA.
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Purity
Usage
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Description

Product Specs

Introduction
TBCA is a tubulin-folding protein involved in the early stages of the tubulin folding pathway. It is one of four proteins (cofactors A, D, E, and C) that guide the formation of properly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to capture and stabilize beta-tubulin in a near-native conformation. TBCA is essential for cell viability. Reduced TBCA levels lead to a decrease in soluble tubulin, microtubule alterations, and G1 cell cycle arrest. Cofactor E binds to the cofactor D-tubulin complex, and subsequent interaction with cofactor C triggers the release of tubulin polypeptides that proceed to their native state.
Formulation
1 mg/ml in PBS, pH 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
Store at 4°C for up to 1 month. For longer storage, store at -20°C. Avoid freeze-thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
TBCA antibody has been validated for ELISA, Western blot, ICC/IF, and FACS analysis to ensure specificity and reactivity. However, optimal working dilutions should be determined experimentally for each application.
Synonyms
Tubulin-specific chaperone A, Tubulin-folding cofactor A, CFA, TCP1-chaperonin cofactor A, TBCA.
Purification Method
TBCA antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1A5AT.
Immunogen
Anti-human TBCA mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human TBCA amino acids 1-108 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.

Product Science Overview

Introduction

Tubulin Folding Cofactor A (TBCA) is a crucial protein involved in the proper folding of beta-tubulin, an essential component of microtubules. Microtubules are part of the cytoskeleton and play a vital role in maintaining cell shape, enabling intracellular transport, and facilitating cell division. TBCA is one of the several cofactors that assist in the folding and assembly of tubulin proteins.

Function and Mechanism

TBCA, along with other cofactors such as TBCD, TBCE, and TBCC, is part of a complex pathway that ensures the correct folding of beta-tubulin from its nascent polypeptide form to its functional state. TBCA specifically captures and stabilizes beta-tubulin intermediates in a quasi-native conformation, preventing their aggregation and misfolding . This process is critical for the formation of functional microtubules, which are essential for various cellular processes.

Mouse Anti-Human TBCA Antibody

The Mouse Anti-Human TBCA antibody is a monoclonal antibody designed to specifically bind to the human TBCA protein. This antibody is used in various research applications, including immunohistochemistry (IHC), western blotting (WB), and immunocytochemistry/immunofluorescence (ICC/IF) . It is a valuable tool for studying the expression and function of TBCA in different biological contexts.

Preparation and Usage

The Mouse Anti-Human TBCA antibody is typically prepared by immunizing mice with a recombinant fragment of the human TBCA protein. The resulting antibodies are then harvested and purified for use in research. This antibody is suitable for detecting TBCA in human samples and can be used in various experimental setups to investigate the role of TBCA in cellular processes .

Applications in Research

Researchers use the Mouse Anti-Human TBCA antibody to study the expression patterns of TBCA in different tissues and under various conditions. This antibody helps in understanding the role of TBCA in microtubule dynamics, cell division, and intracellular transport. Additionally, it can be used to investigate the involvement of TBCA in diseases where microtubule function is compromised, such as neurodegenerative disorders and cancer .

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Source
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