TATDN3 Human

The TATDN3 gene is located on chromosome 1 and is a protein-coding gene. The protein encoded by this gene is known for its DNA nuclease activity, specifically endodeoxyribonuclease activity, which produces 5’-phosphomonoesters . The recombinant form of this protein, often used in research, is typically expressed in E. coli and purified using conventional chromatography techniques .

The recombinant human TATDN3 protein is fused to an N-terminal His-tag, which facilitates its purification and detection. The protein consists of 274 amino acids and has a theoretical molecular weight of approximately 32.9 kDa .

TATDN3 is predicted to be involved in nucleic acid phosphodiester bond hydrolysis, a critical process in DNA metabolism. This activity is essential for various cellular processes, including DNA repair, replication, and recombination . The protein’s nuclease activity suggests it plays a role in maintaining genomic stability by cleaving DNA at specific sites.

TatD DNases, including TATDN3, are conserved across a variety of organisms and are considered potential virulence factors in certain pathogens, such as Plasmodium falciparum and Streptococcus pneumoniae . These proteins contribute to biofilm formation and virulence, highlighting their importance in microbial pathogenicity .

In humans, the precise physiological role of TATDN3 is still under investigation. However, its involvement in DNA metabolism suggests it may play a role in cellular responses to DNA damage and in the regulation of cell cycle progression.

Recombinant human TATDN3 is widely used in research to study its biochemical properties and potential applications in biotechnology and medicine. The protein’s ability to bind metal ions and cleave DNA makes it a valuable tool for understanding DNA repair mechanisms and developing therapeutic strategies for diseases associated with genomic instability.