TATDN1 Human

TatD DNase Domain Containing 1 Human Recombinant
Cat. No.
BT23072
Source
Escherichia Coli.
Synonyms
TatD DNase Domain Containing 1, Hepatocarcinoma High Expression Protein, Putative Deoxyribonuclease TATDN1, EC 3.1.21, CDA11, TATDN1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

TATDN1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-297 a.a) and having a molecular mass of 36kDa. TATDN1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
TatD DNase Domain Containing 1, also known as TATDN1, is a protein coding gene that belongs to the TatD DNase family. TATDN1 is a deoxyribonuclease that binds two divalent metal cations per subunit.
Description
Recombinant human TATDN1 protein was produced in E. coli. It is a single, non-glycosylated polypeptide chain containing 320 amino acids (residues 1-297) with a molecular weight of 36 kDa. The protein has a 23 amino acid His-tag fused to the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
TATDN1 protein solution at a concentration of 1 mg/ml in phosphate buffered saline (pH 7.4) containing 10% glycerol and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity greater than 95% as determined by SDS-PAGE.
Synonyms
TatD DNase Domain Containing 1, Hepatocarcinoma High Expression Protein, Putative Deoxyribonuclease TATDN1, EC 3.1.21, CDA11, TATDN1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSRFKFI DIGINLTDPM FRGIYRGVQK HQDDLQDVIG RAVEIGVKKF MITGGNLQDS KDALHLAQTN GMFFSTVGCH PTRCGEFEKN NPDLYLKELL NLAENNKGKV VAIGECGLDF DRLQFCPKDT QLKYFEKQFE LSEQTKLPMF LHCRNSHAEF LDIMKRNRDR CVGGVVHSFD GTKEAAAALI DLDLYIGFNG CSLKTEANLE VLKSIPSEKL MIETDAPWCG VKSTHAGSKY IRTAFPTKKK WESGHCLKDR NEPCHIIQIL EIMSAVRDED PLELANTLYN NTIKVFFPGI.

Product Science Overview

Gene and Protein Information
  • Gene Name: TATDN1
  • Protein Name: TatD DNase Domain Containing 1
  • Aliases: CDA11, Hepatocarcinoma High Expression Protein, Putative Deoxyribonuclease TATDN1
  • Enzyme Classification: EC 3.1.21.-
Biological Properties

TatD DNase Domain Containing 1 is predicted to enable 3’-5’-exodeoxyribonuclease activity, which means it can degrade DNA by removing nucleotides from the ends of DNA molecules . It is involved in DNA metabolic processes and nucleic acid phosphodiester bond hydrolysis .

Expression Patterns and Tissue Distribution

The TATDN1 gene is expressed in various tissues, with notable expression in the liver and other tissues associated with high metabolic activity . It is also associated with certain cancers, such as lung non-small cell carcinoma and gastric adenosquamous carcinoma .

Biological Functions

TatD DNase Domain Containing 1 plays a crucial role in chromosomal segregation and cell cycle progression, particularly during eye development in zebrafish . This suggests its importance in maintaining genomic stability and proper cell division.

Modes of Action

The protein’s DNase activity allows it to decatenate kinetoplast DNA, which are circular DNA molecules catenated to each other, producing linear DNA molecules . This activity is essential for chromosomal segregation and cell cycle progression.

Regulatory Mechanisms

The regulation of TATDN1 expression and activity is not fully understood. However, its involvement in DNA metabolic processes suggests that it may be regulated by factors that control DNA replication and repair .

Research and Clinical Relevance

TatD DNase Domain Containing 1 is of interest in cancer research due to its association with certain types of cancer . Understanding its function and regulation could provide insights into cancer development and potential therapeutic targets.

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