Introduction
TatD DNase Domain Containing 1, also known as TATDN1, is a protein coding gene that belongs to the TatD DNase family. TATDN1 is a deoxyribonuclease that binds two divalent metal cations per subunit.
Description
Recombinant human TATDN1 protein was produced in E. coli. It is a single, non-glycosylated polypeptide chain containing 320 amino acids (residues 1-297) with a molecular weight of 36 kDa. The protein has a 23 amino acid His-tag fused to the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
TATDN1 protein solution at a concentration of 1 mg/ml in phosphate buffered saline (pH 7.4) containing 10% glycerol and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity greater than 95% as determined by SDS-PAGE.
Synonyms
TatD DNase Domain Containing 1, Hepatocarcinoma High Expression Protein, Putative Deoxyribonuclease TATDN1, EC 3.1.21, CDA11, TATDN1.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSRFKFI DIGINLTDPM FRGIYRGVQK HQDDLQDVIG RAVEIGVKKF MITGGNLQDS KDALHLAQTN GMFFSTVGCH PTRCGEFEKN NPDLYLKELL NLAENNKGKV VAIGECGLDF DRLQFCPKDT QLKYFEKQFE LSEQTKLPMF LHCRNSHAEF LDIMKRNRDR CVGGVVHSFD GTKEAAAALI DLDLYIGFNG CSLKTEANLE VLKSIPSEKL MIETDAPWCG VKSTHAGSKY IRTAFPTKKK WESGHCLKDR NEPCHIIQIL EIMSAVRDED PLELANTLYN NTIKVFFPGI.