Introduction
TRAF Family Member-Associated NFKB Activator (TANK) is found in the cytoplasm and interacts with TRAF1, TRAF2, or TRAF3. TANK acts as a regulator of TRAF function by suppressing TRAF protein activity. This is achieved by sequestering TRAFs in an inactive state within the cytoplasm. When overexpressed, TANK inhibits TRAF2-mediated activation of NF-Kappa-B triggered by CD40 and both TNF receptors. Additionally, it inhibits LMP1-mediated NFkappa-B activation by disrupting the interaction between TRAF2 and LMP1.
Description
Recombinant Human TANK protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 448 amino acids (residues 1-425). It has a molecular weight of 50.2 kDa. A 23 amino acid His-tag is fused to the N-terminus of TANK. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The TANK protein solution is provided at a concentration of 0.5 mg/ml. The solution contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol, and 2mM DTT.
Stability
For optimal storage, keep the TANK protein at 4°C if the entire vial will be used within 2-4 weeks. For prolonged storage periods, freeze the protein at -20°C. To ensure long-term stability during frozen storage, adding a carrier protein such as HSA or BSA to a final concentration of 0.1% is recommended. It's essential to avoid repeated freezing and thawing of the protein.
Purity
The purity of the TANK protein is greater than 85.0% as determined by SDS-PAGE analysis.
Synonyms
TRAF, TRAF2, TRAF-interacting protein, ITRAF.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDKNIGE QLNKAYEAFR QACMDRDSAV KELQQKTENY EQRIREQQEQ LSLQQTIIDK LKSQLLLVNS TQDNNYGCVP LLEDSETRKN NLTLDQPQDK VISGIAREKL PKVRRQEVSS PRKETSARSL GSPLLHERGN IEKTFWDLKE EFHKICMLAK AQKDHLSKLN IPDTATETQC SVPIQCTDKT DKQEALFKPQ AKDDINRGAP SITSVTPRGL CRDEEDTSFE SLSKFNVKFP PMDNDSTFLH STPERPGILS PATSEAVCQE KFNMEFRDNP GNFVKTEETL FEIQGIDPIA SAIQNLKTTD KTKPSNLVNT CIRTTLDRAA CLPPGDHNAL YVNSFPLLDP SDAPFPSLDS PGKAIRGPQQ PIWKPFPNQD SDSVVLSGTD SELHIPRVCE FCQAVFPPSI TSRGDFLRHL NSHFNGET.
