TAFA2 Human
Family with Sequence Similarity 19 Member A2 Human Recombinant
Cat. No.
BT30667
Source
Escherichia Coli.
Synonyms
Family with sequence similarity 19 (chemokine (C-C motif)-like) member A2, Chemokine-like protein TAFA-2, protein FAM19A2.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
TAFA2 Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 101 amino acids and having a molecular mass of 11.2kDa.
The TAFA2 is purified by proprietary chromatographic techniques.
The TAFA2 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
TAFA-2, a member of the FAM19/TAFA chemokine-like protein family, is a secreted protein with a molecular weight of 11 kDa. Characterized by 10 evenly distributed cysteine residues, mature TAFA-1 exhibits a conserved pattern: CX7CCX13CXCX14CX11CX4CX5CX10C (C: conserved cysteine, X: any non-cysteine amino acid). Human TAFA-2 shares a 97% amino acid sequence identity with its mouse counterpart and is found in various tissues including the central nervous system (CNS), colon, heart, lung, spleen, kidney, and thymus. Notably, its expression in the CNS is significantly higher (50 to 1000 fold) compared to other tissues. The specific biological functions of TAFA family members are currently being explored.
Description
Recombinant Human TAFA2, expressed in E.Coli, is a non-glycosylated polypeptide chain comprising 101 amino acids, resulting in a molecular weight of 11.2 kDa. The purification of TAFA2 is achieved using proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
TAFA2 was lyophilized from a 0.2µm filtered concentrated solution prepared in 1xPBS, pH 7.4.
Solubility
To reconstitute lyophilized TAFA2, sterile 18M-cm H₂O is recommended, aiming for a concentration not less than 100µg/ml. This solution can be further diluted into other aqueous solutions as needed.
Stability
Lyophilized TAFA2 exhibits stability at room temperature for up to 3 weeks; however, it is recommended to store it desiccated below -18°C. After reconstitution, TAFA2 should be stored at 4°C for a period of 2-7 days. For long-term storage, it is advisable to store it below -18°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. It's important to avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 97.0% as determined by (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
Demonstrates full biological activity in comparison to the established standard. Activity is evaluated based on its capacity to promote neurite outgrowth in E16-E18 rat embryonic cortical neurons.
Synonyms
Family with sequence similarity 19 (chemokine (C-C motif)-like) member A2, Chemokine-like protein TAFA-2, protein FAM19A2.
Source
Escherichia Coli.
Amino Acid Sequence
ANHHKAHHVK TGTCEVVALH RCCNKNKIEE RSQTVKCSCF PGQVAGTTRA APSCVDASIV EQKWWCHMQP CLEGEECKVL PDRKGWSCSS GNKVKTTRVT H
Product Science Overview
Gene and Protein Structure
The FAM19A2 gene encodes a protein that is 101 amino acids long and has a molecular mass of approximately 11.2 kDa . The recombinant human TAFA2 protein is often produced in E. coli as a non-glycosylated polypeptide . In some cases, it is expressed with an Fc region of human IgG1 at the N-terminus, resulting in a disulfide-linked homodimer with a predicted molecular mass of 39.7 kDa .
Expression Patterns and Tissue Distribution
Biological Functions and Hypotheses
The exact biological functions of TAFA family members, including FAM19A2, are still under investigation. However, several hypotheses have been proposed :
- Immune Modulation in the CNS: TAFAs may act as brain-specific chemokines, optimizing the recruitment and activity of immune cells in the CNS.
- Neurokine Activity: TAFAs might represent a novel class of neurokines that regulate immune nervous cells.
- Axonal Sprouting: TAFAs could control axonal sprouting following brain injury.
Recombinant Protein Production
Recombinant human TAFA2 is produced using various expression systems, including E. coli and HEK293 cells . The protein is typically purified using proprietary chromatographic techniques to ensure high purity and biological activity . It is often provided as a lyophilized powder for stability and ease of storage .
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