TAF15 Human

TAF15 Human Recombinant

Cat. No.

BT22905

Source

Escherichia Coli.

Synonyms

Npl3, RBP56, TAF2N, TAFII68, TATA-binding protein-associated factor 2N, 68 kDa TATA-binding protein-associated factor, RNA-binding protein 56, TAF15.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

TAF15 Human Recombinant produced in E. coli is. a single polypeptide chain containing 282 amino acids (148-406) and having a molecular mass of 30kDa. TAF15 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

As a member of the TET family, the RNA-binding protein TAF15 plays a crucial role in RNA polymerase II gene transcription as a component of specific multi-subunit transcription initiation factor TFIID complexes. Notably, TAF15 is implicated in severe leukemia and extraskeletal myxoid chondrosarcoma due to translocations. Furthermore, mutations in TAF15 have been linked to amyotrophic lateral sclerosis.

Description

Recombinant human TAF15, produced in E. coli, is a single polypeptide chain with a molecular weight of 30kDa. It comprises 282 amino acids (residues 148-406) and is fused to a 23 amino acid His-tag at the N-terminus. Purification is achieved through proprietary chromatographic techniques.

Physical Appearance

The product is a clear solution that has been sterilized through filtration.

Formulation

The TAF15 solution is provided at a concentration of 0.25mg/ml and is formulated in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 30% glycerol, and 1mM DTT.

Stability

For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Importantly, repeated freezing and thawing should be avoided.

Purity

Analysis by SDS-PAGE indicates a purity exceeding 90%.

Synonyms

Npl3, RBP56, TAF2N, TAFII68, TATA-binding protein-associated factor 2N, 68 kDa TATA-binding protein-associated factor, RNA-binding protein 56, TAF15.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSSYHSQRE NYSHHTQDDR RDVSRYGEDN RGYGGSQGGG RGRGGYDKDG RGPMTGSSGG DRGGFKNFGG HRDYGPRTDA DSESDNSDNN TIFVQGLGEG VSTDQVGEFF KQIGIIKTNK KTGKPMINLY TDKDTGKPKG EATVSFDDPP SAKAAIDWFD GKEFHGNIIK VSFATRRPEF MRGGGSGGGR RGRGGYRGRG GFQGRGGDPK SGDWVCPNPS CGNMNFARRN SCNQCNEPRP EDSRPSGGDF RGRGYGGERG YR.

Product Science Overview

Structure and Function

TAF15 is a member of the FET (FUS, EWS, TAF15) family of proteins, which are structurally similar and multifunctional. These proteins were initially discovered due to their involvement in fusion oncogenes in human sarcomas and leukemias. TAF15 contains RNA recognition motifs (RRMs) and glycine-rich regions, which enable it to bind RNA and DNA.

The primary function of TAF15 is to facilitate the assembly of the transcription complex by interacting with other transcription factors and coactivators. It plays a crucial role in the regulation of gene expression by binding to the core promoter region of genes and positioning RNA polymerase II correctly. Additionally, TAF15 is involved in various cellular processes, including RNA splicing, transport, and stability.

Recombinant TAF15

Recombinant TAF15 is a form of the protein that is produced using recombinant DNA technology. This involves inserting the TAF15 gene into an expression vector, which is then introduced into a host cell (such as E. coli) to produce the protein. The recombinant protein can be purified and used for various research applications, including studying its function, interactions, and role in diseases.

The recombinant TAF15 protein typically includes a His-tag at the N-terminus to facilitate purification. It corresponds to the amino acids 148-406 of the human TAF15 protein.

Clinical Significance

TAF15 has been implicated in several diseases, particularly cancers. Translocations involving the TAF15 gene and other genes, such as the nuclear receptor CSMF, result in fusion proteins that are associated with a subset of extraskeletal myxoid chondrosarcomas. These fusion proteins can disrupt normal cellular functions and contribute to tumorigenesis.

Furthermore, TAF15 is involved in the regulation of transcription by RNA polymerase II, which is a critical process in maintaining cellular homeostasis and responding to various signals. Dysregulation of this process can lead to various diseases, including cancer.

Research Applications

Recombinant TAF15 is widely used in research to study its role in transcription regulation, RNA binding, and its involvement in diseases. It is also used to investigate the interactions between TAF15 and other proteins, as well as its function in the context of the TFIID complex.

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TAF15 Human

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