TADA3 Human

The recombinant human TADA3 protein is typically expressed in Escherichia coli and is often tagged with a His-tag at the N-terminus for purification purposes . The protein consists of 432 amino acids and has a theoretical molecular weight of approximately 51.3 kDa .

TADA3 functions as a transcriptional activator adaptor. It plays a significant role in the acetylation of histones, which is a key process in the regulation of gene expression. The PCAF complex, in which TADA3 is a component, is capable of efficiently acetylating histones in a nucleosomal context . This acetylation process is crucial for the activation of transcription.

Additionally, TADA3 is known to associate with the tumor suppressor protein p53. This association is essential for the full activity of p53 and p53-mediated apoptosis . The interaction with p53 highlights TADA3’s role in cellular processes such as DNA repair, cell cycle regulation, and apoptosis.

The involvement of TADA3 in histone acetylation and its interaction with p53 underscore its importance in maintaining cellular homeostasis and regulating gene expression. Dysregulation of TADA3 or its associated complexes can lead to aberrant gene expression and has been implicated in various diseases, including cancer.

Recombinant TADA3 protein is widely used in research to study its function and interactions. It is utilized in assays to understand the mechanisms of transcriptional regulation and the role of histone acetylation in gene expression. The recombinant form allows for detailed biochemical and structural studies, providing insights into its function and potential therapeutic targets.