SYNJ2BP Human

Synaptojanin 2 Binding Protein Human Recombinant
Cat. No.
BT22389
Source
Escherichia Coli.
Synonyms
Synaptojanin 2 binding protein, ARIP2, OMP25, Synaptojanin-2-binding protein, Mitochondrial outer membrane protein 25, SYNJ2BP.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

SYNJ2BP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids (1-117 a.a) and having a molecular mass of 15.0kDa.
SYNJ2BP is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Synaptojanin 2 Binding Protein (SYNJ2BP) contains a PDZ domain, also known as a DHR domain. This domain enables SYNJ2BP to bind to isoform 2A of SYNJ2 through a unique motif located in the C-terminus of SYNJ2. Additionally, SYNJ2BP interacts with MAPK12.
Description
Recombinant human SYNJ2BP, expressed in E. coli, is a single polypeptide chain lacking glycosylation. It comprises 140 amino acids, with amino acids 1-117 representing the SYNJ2BP sequence. The protein has a molecular mass of 15.0 kDa. For purification and detection purposes, a 23 amino acid His-tag is fused to the N-terminus of SYNJ2BP. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution that has been filtered for sterility.
Formulation
The SYNJ2BP protein solution has a concentration of 1 mg/ml and is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA. It is important to avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of SYNJ2BP is determined by SDS-PAGE analysis and is greater than 95.0%.
Synonyms
Synaptojanin 2 binding protein, ARIP2, OMP25, Synaptojanin-2-binding protein, Mitochondrial outer membrane protein 25, SYNJ2BP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMNGRVDY LVTEEEINLT RGPSGLGFNI VGGTDQQYVS NDSGIYVSRI KENGAAALDG RLQEGDKILS VNGQDLKNLL HQDAVDLFRN AGYAVSLRVQ HRLQVQNGPI GHRGEGDPSG.

Product Science Overview

Structure and Localization

SYNJ2BP contains a PDZ domain, which is crucial for its interaction with other proteins. This domain allows SYNJ2BP to bind to the C-terminal PDZ domain-binding motif of its interaction partners . The protein is predominantly localized in the mitochondria and the plasma membrane, where it plays a role in cellular signaling and adhesion .

Function and Mechanism

SYNJ2BP has been shown to interact with the cell adhesion molecule TMIGD1. This interaction is mediated by the PDZ domain of SYNJ2BP and the C-terminal PDZ domain-binding motif of TMIGD1 . SYNJ2BP can actively recruit TMIGD1 to mitochondria, providing a potential mechanism for the localization of TMIGD1 at mitochondria .

In addition to its role in cellular adhesion, SYNJ2BP is involved in the regulation of sprouting angiogenesis, a process critical for the formation of new blood vessels . This function is particularly important in the context of cancer, where SYNJ2BP has been shown to inhibit tumor growth and metastasis by activating the DLL4-mediated Notch signaling pathway .

Clinical Significance

SYNJ2BP has been implicated in various diseases, including hepatocellular carcinoma (HCC) and Loeys-Dietz Syndrome 4 . In HCC, SYNJ2BP expression is decreased, and its low expression is associated with poor prognosis . Studies have shown that SYNJ2BP inhibits HCC cell invasion, migration, and proliferation, suggesting its potential as a therapeutic target .

Research and Applications

The recombinant form of SYNJ2BP is used in various research applications to study its function and interactions. Understanding the role of SYNJ2BP in cellular processes and disease mechanisms can provide insights into potential therapeutic strategies for conditions such as cancer and genetic disorders.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.