SVIP Human
Description
SVIP is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Small VCP/P97-Interacting Protein (SVIP) is a crucial adaptor protein localized on the cytosolic surface of the endoplasmic reticulum (ER) membrane. It plays a significant role in the endoplasmic reticulum-associated degradation (ERAD) pathway by inhibiting the activity of p97/VCP, a protein involved in various cellular processes such as cell cycle regulation, post-mitotic membrane fusion, and ubiquitination of misfolded proteins .
SVIP is characterized by its ability to directly interact with p97/VCP. It is predominantly found on the cytosolic surface of the ER membrane, where it exerts its inhibitory effects on p97/VCP. This interaction is crucial for maintaining the balance of protein degradation within the ER, ensuring that misfolded proteins are properly managed .
SVIP has been identified as an androgen-responsive gene, indicating its regulation by androgens. It plays a pivotal role in the ERAD pathway, which is responsible for the degradation of misfolded proteins within the ER. By inhibiting p97/VCP, SVIP helps regulate the degradation process, preventing the accumulation of misfolded proteins that could otherwise lead to cellular stress and dysfunction .
Recent studies have highlighted the involvement of SVIP in steroidogenesis, particularly in Leydig cells. Leydig cells are responsible for the production of testosterone, a process that involves several key proteins such as StAR, CYP17A1, and 3β-HSD. Research has shown that SVIP, along with p97/VCP, colocalizes with these proteins in Leydig cells, suggesting a regulatory role in steroidogenesis .
Experimental studies using Leydig cell lines (HLC, TM3, and MA-10) have demonstrated that the suppression of SVIP and p97/VCP using siRNAs leads to a decrease in the expression of StAR, CYP17A1, and 3β-HSD. This suppression also results in reduced testosterone levels, indicating that SVIP and p97/VCP are essential for the proper synthesis of testosterone. Additionally, it was observed that SVIP expression increases in response to hCG stimulation, further supporting its role in steroidogenesis .
