SUGT1 Human
SGT1 Recombinant Human
Cat. No.
BT22064
Source
Escherichia Coli.
Synonyms
SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae), SGT1, Putative 40-6-3 protein, SGT1B protein.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
SGT1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (115-365.a.a) and having a molecular mass of 30.7kDa.
SGT1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SGT1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
SUGT1, a homolog of the yeast protein SGT1, is a cell cycle regulator crucial for G1/S and G2/M transitions. This protein contains a CS domain, an SGS domain, a p23 domain, and three tetratricopeptide repeats (TPRs). SUGT1 interacts with Skp1 p19 and CUL-1, components of the SCF ubiquitin ligase complex, suggesting its involvement in protein degradation. Additionally, SUGT1 is essential for kinetochore assembly and acts as a co-chaperone for HSP90.
Description
Produced in E. coli, SGT1 is a single, non-glycosylated polypeptide chain consisting of 252 amino acids (115-365.a.a) with a molecular weight of 30.7 kDa. This SGT1 protein is fused to a 21 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The SGT1 protein solution (0.5 mg/mL) is formulated in 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 1 mM DTT, and 20% glycerol.
Purity
Greater than 90% purity as determined by SDS-PAGE.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Avoid repeated freeze-thaw cycles.
Synonyms
SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae), SGT1, Putative 40-6-3 protein, SGT1B protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MHRVGQAGLQ LLTSSDPPAL DSQSAGITGA DANFSVWIKR CQEAQNGSES EVWTHQSKIK YDWYQTESQVVITLMIKNVQ KNDVNVEFSE KELSALVKLP SGEDYNLKLE LLHPIIPEQS TFKVLSTKIE IKLKKPEAVR WEKLEGQGDV PTPKQFVADVKNLYPSSSPY TRNWDKLVGE IKEEEKNEKL EGDAALNRLF QQIYSDGSDE VKRAMNKSFM ESGGTVLSTN WSDVGKRKVE INPPDDMEWKKY
Product Science Overview
Structure and Isoforms
In humans, the SGT1 gene encodes two main isoforms, SGT1A and SGT1B, which differ by 33 amino acids in the TPR (tetratricopeptide repeat) domain . There is also a third isoform, SGT1C, which has a distinct N-terminus and is shorter than the other two isoforms . The functional significance of SGT1C is not yet fully understood.
Functions
SGT1 functions as a co-chaperone with HSP90 (Heat Shock Protein 90), assisting in the proper folding and stabilization of various client proteins . It is involved in several biological processes, including:
- Innate Immune Systems: SGT1 plays a role in both plant and mammal innate immune responses .
- Kinetochore Assembly: It is essential for the assembly of the kinetochore complex, which is critical for accurate chromosome segregation .
- SCF Function and Cyclic AMP Signaling: SGT1 is involved in the SCF (Skp, Cullin, F-box containing complex) ubiquitin ligase activity and cyclic AMP signaling pathways .
- Centrosome Organization and Cytokinesis: It contributes to the organization of centrosomes and the process of cytokinesis .
- Brain and Heart Diseases: SGT1 has been implicated in the development of certain brain and heart diseases .
Recombinant Human SGT1
Clinical Implications
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