SUFU Human

Suppressor of Fused Homolog Human Recombinant
Cat. No.
BT21988
Source
Escherichia Coli.
Synonyms
Suppressor of fused homolog, SUFUH, SUFU, UNQ650/PRO1280, SUFUXL, PRO1280.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SUFU Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 504 amino acids (1-484 a.a.) and having a molecular mass of 56.1kDa.
SUFU is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Suppressor of fused homolog (SUFU) is a negative regulator in the hedgehog signaling pathway and belongs to the SUFU family. This protein interacts with GLI1, GLI3, and PEX26. Defects in the SUFU gene can lead to medulloblastoma.
Description
Recombinant human SUFU protein, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 504 amino acids (1-484 a.a.) with a molecular weight of 56.1 kDa. This protein is purified using proprietary chromatographic techniques and is fused to a 20 amino acid His-tag at the N-terminus.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
The SUFU protein solution is provided at a concentration of 0.5mg/ml and contains the following components: 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 10% glycerol, and 50mM NaCl.
Stability
For short-term storage (up to 2-4 weeks), the solution can be stored at 4°C. For longer periods, it is recommended to store the solution frozen at -20°C. To ensure long-term stability, consider adding a carrier protein such as 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of this protein is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
Suppressor of fused homolog, SUFUH, SUFU, UNQ650/PRO1280, SUFUXL, PRO1280.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAELRPSGAP GPTAPPAPGP TAPPAFASLF PPGLHAIYGE CRRLYPDQPN PLQVTAIVKY WLGGPDPLDY VSMYRNVGSP SANIPEHWHY ISFGLSDLYG DNRVHEFTGT DGPSGFGFEL TFRLKRETGE SAPPTWPAEL MQGLARYVFQ SENTFCSGDH VSWHSPLDNS ESRIQHMLLT EDPQMQPVQT PFGVVTFLQI VGVCTEELHS AQQWNGQGIL ELLRTVPIAG GPWLITDMRR GETIFEIDPH LQERVDKGIE TDGSNLSGVS AKCAWDDLSR PPEDDEDSRS ICIGTQPRRL SGKDTEQIRE TLRRGLEINS KPVLPPINPQ RQNGLAHDRA PSRKDSLESD SSTAIIPHEL IRTRQLESVH LKFNQESGAL IPLCLRGRLL HGRHFTYKSI TGDMAITFVS TGVEGAFATE EHPYAAHGPW LQILLTEEFV EKMLEDLEDL TSPEEFKLPK EYSWPEKKLK VSILPDVVFD SPLH.

Product Science Overview

Structure and Function

SUFU is a negative regulator of the Hedgehog signaling pathway. It interacts with the GLI family of transcription factors, particularly GLI1, to inhibit their activity and prevent the transcription of target genes . The human recombinant form of SUFU is often produced in E. coli and includes an N-terminal His-tag for purification purposes .

Role in Disease

Mutations or defects in the SUFU gene can lead to various medical conditions. One notable condition is medulloblastoma, a type of brain cancer that primarily affects children . The loss of SUFU function can result in uncontrolled cell growth due to the dysregulation of the Hedgehog signaling pathway.

Applications in Research

Recombinant SUFU proteins are widely used in research to study the Hedgehog signaling pathway and its implications in various diseases. These proteins are utilized in antibody competition assays to confirm antibody specificity . They are also used in structural and functional studies to understand the interactions between SUFU and other components of the Hedgehog pathway.

Storage and Handling

Recombinant SUFU proteins are typically stored at -20°C to maintain their stability and activity. It is important to avoid freeze-thaw cycles to prevent protein degradation .

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