STIM1 Human

Stromal Interaction Molecule 1 Human Recombinant
Cat. No.
BT21450
Source
Escherichia Coli.
Synonyms
Stromal interaction molecule 1, GOK, D11S4896E, STIM-1, STIM1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant STIM1 produced in E.Coli is a single, non-glycosylated polypeptide chain (aa 23-213) containing a total of 343 amino acids and having a molecular mass of 38kDa.
STIM1 is fused to a 152 aa Calmodulin tag at N-terminus and purified by conventional chromatography techniques.

Product Specs

Introduction
STIM1, a type-1 transmembrane protein, plays a critical role in store-operated Ca(2+) entry, facilitating extracellular Ca(2+) influx in response to depleted endoplasmic reticulum (ER) Ca(2+) stores. Primarily located in the ER, STIM1 relocates to ER-plasma membrane junctions upon Ca(2+) release from the ER, activating Ca(2+) channels. Functioning as an adhesion molecule, STIM1 participates in early hematopoiesis by mediating stromal cell attachment. It also influences the survival and/or proliferation of b-cell precursors. Notably, the STIM1 gene resides within the imprinted gene domain of 11p15.5, a crucial tumor-suppressor gene region. Alterations in this region are linked to disorders like Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and various cancers. Beyond its role in early hematopoiesis, STIM1's involvement in malignancies is attributed to its stromal cell attachment mediation. Interestingly, STIM1 exhibits a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with its 3' end situated 1.6 kb from the RRM1 gene's 5' end.
Description
Recombinant STIM1, expressed in E.Coli, is a single, non-glycosylated polypeptide chain encompassing amino acids 23-213. With a total of 343 amino acids, it possesses a molecular mass of 38kDa. This STIM1 variant is fused to a 152 amino acid Calmodulin tag at its N-terminus and undergoes purification using standard chromatography methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The STIM1 protein solution is provided at a concentration of 1mg/ml in a buffer containing 20mM Tris-HCl at pH 7.5.
Stability
For optimal storage, refrigerate the entire vial at 4°C if it will be consumed within 2-4 weeks. For extended storage, freeze the solution at -20°C. To ensure long-term stability, adding a carrier protein like HSA or BSA (0.1%) is recommended. It's crucial to avoid repeated freeze-thaw cycles.
Purity
The purity of this product exceeds 90.0%, as determined by SDS-PAGE analysis.
Synonyms
Stromal interaction molecule 1, GOK, D11S4896E, STIM-1, STIM1.
Source
Escherichia Coli.
Amino Acid Sequence
MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SMLSHSHSEK ATGTSSGANS EESTAAEFCR IDKPLCHSED EKLSFEAVRN IHKLMDDDAN GDVDVEESDE FLREDLNYHD PTVKHSTFHG EDKLISVEDL WKAWKSSEVY NWTVDEVVQW LITYVELPQY EETFRKLQLS GHAMPRLAVT NTTMTGTVLK MTDRSHRQKL QLKALDTVLF GPPLLTRHNH LKD.

Product Science Overview

Introduction

Stromal Interaction Molecule 1 (STIM1) is a protein encoded by the STIM1 gene in humans. It plays a crucial role in cellular calcium signaling, particularly in the regulation of store-operated calcium entry (SOCE). This protein is primarily localized in the endoplasmic reticulum (ER) and, to a lesser extent, in the plasma membrane .

Structure and Localization

STIM1 has a single transmembrane domain and is characterized by its ability to sense calcium levels within the ER. The protein contains an EF-hand domain that binds calcium ions. When the calcium concentration in the ER decreases, STIM1 undergoes a conformational change, leading to its oligomerization and translocation to regions of the ER that are close to the plasma membrane .

Function

The primary function of STIM1 is to act as a calcium sensor within the ER. Upon sensing a decrease in calcium levels, STIM1 clusters and interacts with ORAI1 channels in the plasma membrane, facilitating calcium influx into the cell. This process is essential for various cellular functions, including gene expression, cell migration, and the regulation of the cell cycle .

Role in DNA Damage Response

Recent studies have uncovered a novel function of STIM1 in the DNA damage response. STIM1 translocates to the nucleus in response to DNA damage, where it helps protect cells from endogenous DNA damage and replicative stress. This nuclear function of STIM1 is crucial for maintaining genomic integrity and ensuring proper DNA repair mechanisms .

Clinical Significance

Mutations or dysregulation of STIM1 have been associated with several diseases, including immune deficiencies and muscle disorders. Understanding the role of STIM1 in calcium signaling and DNA damage response can provide insights into potential therapeutic targets for these conditions .

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