STEAP4 Human

STEAP Family Member 4 Human Recombinant
Cat. No.
BT21380
Source
Escherichia Coli.
Synonyms
DKFZp666D049, FLJ23153, STAMP2, TIARP, TNFAIP9, Six-transmembrane epithelial antigen of prostate 4, SixTransMembrane protein of prostate 2, Tumor necrosis factor, alpha-induced protein 9.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

STEAP4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 188 amino acids (1-152 a.a) and having a molecular mass of 20.6kDa.STEAP4 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
STEAP Family Member 4 (STEAP4), a member of the STEAP (six transmembrane epithelial antigen of prostate) family, is located in the Golgi apparatus. It functions as a metalloreductase with the capability to reduce both ferric iron (Fe3+) to ferrous iron (Fe2+) and cupric ions (Cu2+) to cuprous ions (Cu1+), utilizing NAD+ as an electron acceptor. Studies conducted on mice and humans suggest that STEAP4 may be involved in adipocyte development and metabolism, as well as contribute to the normal physiological functions of prostate cells.
Description
Recombinant human STEAP4, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 188 amino acids (specifically, amino acids 1 to 152). It has a molecular weight of 20.6 kDa. The protein includes a 36-amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
The product is a clear, sterile-filtered solution.
Formulation
The STEAP4 protein solution is provided at a concentration of 1 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.4 M urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Avoid repeated cycles of freezing and thawing.
Purity
The purity of the STEAP4 protein is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
DKFZp666D049, FLJ23153, STAMP2, TIARP, TNFAIP9, Six-transmembrane epithelial antigen of prostate 4, SixTransMembrane protein of prostate 2, Tumor necrosis factor, alpha-induced protein 9.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMEKT CIDALPLTMN SSEKQETVCI FGTGDFGRSL GLKMLQCGYS VVFGSRNPQK TTLLPSGAEV LSYSEAAKKS GIIIIAIHRE HYDFLTELTE VLNGKILVDI SNNLKINQYP ESNAEYLAHL VPGAHVVKAF NTISAWALQS GALDASRQ.

Product Science Overview

Introduction

STEAP (Six-Transmembrane Epithelial Antigen of Prostate) family proteins are a group of metalloreductases involved in metal ion metabolism. The family includes four members: STEAP1, STEAP2, STEAP3, and STEAP4. These proteins are unique to mammals and play significant roles in various biological processes, including molecular trafficking, cell proliferation, and apoptosis .

STEAP4 Overview

STEAP4, also known as Six-Transmembrane Epithelial Antigen of Prostate 4, is a protein encoded by the STEAP4 gene in humans. It is highly expressed in several tissues, including adipose tissue, bone marrow, heart, lung, placenta, and prostate . STEAP4 is known for its iron reductase activity, which contributes to the regulation of reactive oxygen species (ROS) levels within cells .

Biological Functions

STEAP4 functions primarily as a metalloreductase, facilitating the reduction of iron and copper ions. This activity is crucial for maintaining metal homeostasis within cells. The protein’s role in reducing iron and copper ions is mediated by its conserved histidine residues, which bind to an intramembrane heme group .

In addition to its metalloreductase activity, STEAP4 is involved in various cellular processes:

  • Cell Proliferation and Apoptosis: STEAP4 influences cell proliferation and apoptosis through its impact on ROS levels. Increased ROS levels can lead to oxidative stress, which in turn affects cell survival and proliferation .
  • Cancer: STEAP4 is overexpressed in several types of cancers, including prostate, bladder, and gastric cancers. Its expression levels are associated with cancer progression and patient prognosis .
Recombinant STEAP4

Human recombinant STEAP4 is produced using recombinant DNA technology, which involves inserting the STEAP4 gene into a suitable expression system, such as bacteria or mammalian cells. This allows for the production of large quantities of the protein for research and therapeutic purposes.

Applications in Research and Medicine

The study of STEAP4 has significant implications for understanding metal metabolism and its role in various diseases. Some key applications include:

  • Cancer Research: Given its overexpression in several cancers, STEAP4 is a potential biomarker and therapeutic target. Research is ongoing to develop STEAP4-targeted therapies for cancer treatment .
  • Metabolic Disorders: STEAP4’s role in metal ion metabolism makes it a candidate for studying metabolic disorders related to iron and copper homeostasis .

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