STAP1 Human

Signal Transducing Adaptor Family Member 1 Human Recombinant
Cat. No.
BT21104
Source
Escherichia Coli.
Synonyms
Signal-transducing adaptor protein 1, STAP-1, BCR downstream-signaling protein 1, Docking protein BRDG1, Stem cell adaptor protein 1, STAP1, BRDG1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

STAP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 319 amino acids (1-295 a.a) and having a molecular mass of 36.8kDa.
STAP1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Signal-transducing adaptor protein 1 (STAP1) is an important molecule involved in how B cells, a type of immune cell, receive and respond to signals. Specifically, STAP1 helps transmit signals from a protein called Tec, which is activated when the B cell receptor on the surface of the B cell recognizes a foreign antigen. STAP1 acts as a link in this signaling chain, helping to amplify the signal and activate downstream processes involved in the immune response.
Description
This product consists of the human STAP1 protein, manufactured in a laboratory using E. coli bacteria. It is engineered to be a single chain of 319 amino acids, representing the key functional part of the natural protein. This recombinant STAP1 protein is not glycosylated (meaning it lacks sugar modifications typically found in proteins) and has a molecular weight of 36.8 kDa. For purification and ease of handling, it includes a 24 amino acid His-tag attached to its beginning (N-terminus).
Physical Appearance
The product is a clear and colorless liquid that has been sterilized by filtration.
Formulation
The STAP1 protein is supplied in a solution containing 1mg of protein per ml. The solution is buffered with 20mM Tris-HCl at a pH of 8.0 and also includes 30% glycerol as a stabilizing agent, 0.1M NaCl for maintaining proper ionic strength, and 1mM DTT as a reducing agent to prevent unwanted protein interactions.
Stability
To ensure product stability, keep the vial refrigerated at 4°C if using within 2-4 weeks. For longer storage, freeze at -20°C. It's recommended to add a carrier protein like HSA or BSA (0.1%) for extended storage to further protect the protein. Avoid repeated freezing and thawing.
Purity
This STAP1 protein product is highly pure, exceeding 95% purity as assessed by SDS-PAGE, a standard method for determining protein purity.
Synonyms
Signal-transducing adaptor protein 1, STAP-1, BCR downstream-signaling protein 1, Docking protein BRDG1, Stem cell adaptor protein 1, STAP1, BRDG1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMMAKKP PKPAPRRIFQ ERLKITALPL YFEGFLLIKR SGYREYEHYW TELRGTTLFF YTDKKSIIYV DKLDIVDLTC LTEQNSTEKN CAKFTLVLPK EEVQLKTENT ESGEEWRGFI LTVTELSVPQ NVSLLPGQVI KLHEVLEREK KRRIETEQST SVEKEKEPTE DYVDVLNPMP ACFYTVSRKE ATEMLQKNPS LGNMILRPGS DSRNYSITIR QEIDIPRIKH YKVMSVGQNY TIELEKPVTL PNLFSVIDYF VKETRGNLRP FICSTDENTG QEPSMEGRSE KLKKNPHIA.

Product Science Overview

Gene and Protein Structure

The STAP1 gene is located on chromosome 4q13.2 . The protein encoded by this gene contains several important domains:

  • Proline-rich region: This region is involved in protein-protein interactions.
  • Pleckstrin Homology (PH) domain: This domain is known for its role in binding phosphoinositides and is crucial for membrane localization.
  • Src Homology 2 (SH2) domain: This domain is involved in binding phosphorylated tyrosine residues, which is essential for signal transduction.
Function and Mechanism

STAP1 functions primarily as a docking protein in BCR (B-cell receptor) signaling pathways. It acts downstream of the Tec tyrosine kinase and participates in a positive feedback loop by enhancing the activity of Tec . This interaction is phosphorylation-dependent, meaning that the binding and activity of STAP1 are regulated by the addition of phosphate groups to tyrosine residues on the protein .

Biological Significance

STAP1 is predominantly expressed in the spleen and lymph nodes, indicating its significant role in the immune system . It has been implicated in various intracellular signaling pathways, including the EPO-induced Jak-STAT pathway, which is crucial for erythropoiesis (the production of red blood cells) .

Clinical Relevance

Variants of the STAP1 gene have been associated with autosomal-dominant hypercholesterolemia (ADH), a condition characterized by elevated levels of low-density lipoprotein (LDL) cholesterol and an increased risk of coronary vascular disease . This makes STAP1 a potential target for therapeutic interventions aimed at managing cholesterol levels and preventing cardiovascular diseases.

Research and Applications

Recombinant human STAP1 is used in various research applications to study its role in signal transduction and its potential implications in diseases. Understanding the function and regulation of STAP1 can provide insights into the development of targeted therapies for conditions associated with its dysregulation.

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