STAMBPL1 Human
STAM Binding Protein Like 1 Human Recombinant
Cat. No.
BT21015
Source
Escherichia Coli.
Synonyms
ALMalpha, AMSH-FP, AMSH-LP, bA399O19.2, AMSH-like protease, STAM Binding Protein Like 1, STAMBPL1, AMSHLP, KIAA1373.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
STAMBPL1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 458 amino acids (1-436) and having a molecular mass of 52 kDa. STAMBPL1 is fused to a 22 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
STAM Binding Protein Like 1 (STAMBPL1) is a zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. However, it does not cleave 'Lys-48'-linked polyubiquitin chains. Each subunit of STAMBPL1 binds to two zinc ions. The JAMM motif is essential for its protease activity. STAMBPL1 belongs to the peptidase M67C family and contains one MPN (JAB/Mov34) domain.
Description
Recombinant human STAMBPL1, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 458 amino acids (residues 1-436) and has a molecular weight of 52 kDa. The STAMBPL1 protein is fused to a 22 amino acid His-tag at its N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The STAMBPL1 solution has a concentration of 0.5 mg/ml and is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability, adding a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 90.0%, as determined by SDS-PAGE analysis.
Synonyms
ALMalpha, AMSH-FP, AMSH-LP, bA399O19.2, AMSH-like protease, STAM Binding Protein Like 1, STAMBPL1, AMSHLP, KIAA1373.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSQ SHMDQPFTVN SLKKLAAMPD HTDVSLSPEE RVRALSKLGC NITISEDITP RRYFRSGVEM ERMASVYLEE GNLENAFVLY NKFITLFVEK LPNHRDYQQC AVPEKQDIMK KLKEIAFPRT DELKNDLLKK YNVEYQEYLQ SKNKYKAEIL KKLEHQRLIE AERKRIAQMR QQQLESEQFL FFEDQLKKQE LARGQMRSQQ TSGLSEQIDG SALSCFSTHQ NNSLLNVFAD QPNKSDATNY ASHSPPVNRA LTPAATLSAV QNLVVEGLRC VVLPEDLCHK FLQLAESNTV RGIETCGILC GKLTHNEFTI THVIVPKQSA GPDYCDMENV EELFNVQDQH DLLTLGWIHT HPTQTAFLSS VDLHTHCSYQ LMLPEAIAIV CSPKHKDTGI FRLTNAGMLE VSACKKKGFH PHTKEPRLFS ICKHVLVKDI KIIVLDLR.
Product Science Overview
Structure and Function
STAMBPL1 encodes a zinc metalloprotease that specifically cleaves Lys63-linked polyubiquitin chains . This deubiquitinating enzyme (DUB) is involved in the removal of ubiquitin moieties from target proteins, a process crucial for maintaining protein homeostasis within the cell . Unlike other DUBs, STAMBPL1 does not cleave Lys48-linked polyubiquitin chains .
One of the primary functions of STAMBPL1 is its role as a positive regulator of the TORC1 signaling pathway. It mediates the deubiquitination of SESN2, thereby inhibiting SESN2’s interaction with the GATOR2 complex . This regulation is essential for cellular growth and metabolism.
Biological Significance
STAMBPL1 is involved in several critical cellular processes, including:
- Protein Degradation: By cleaving Lys63-linked polyubiquitin chains, STAMBPL1 helps in the degradation of misfolded or damaged proteins, thus maintaining cellular protein quality control .
- Cell Signaling: It plays a role in the TORC1 signaling pathway, which is vital for cell growth, proliferation, and metabolism .
- Epithelial-Mesenchymal Transition (EMT): Recent studies have shown that STAMBPL1 is involved in the EMT process, which is crucial for cancer progression and metastasis . It regulates the transcription factor SNAI1, affecting the migratory capacity of cancer cells .
Clinical Significance
STAMBPL1 has been associated with various diseases and conditions:
- Cancer: Overexpression of STAMBPL1 has been linked to the progression and dissemination of multiple carcinomas, including lung and breast cancers . Its expression is regulated by mutant p53, and its catalytic activity is required for the EMT process .
- Aortic Aneurysm: Mutations in the STAMBPL1 gene have been associated with aortic aneurysm, a condition characterized by the abnormal bulging of the aorta .
- Hermansky-Pudlak Syndrome 2: This rare genetic disorder, which affects multiple systems in the body, has also been linked to mutations in the STAMBPL1 gene .
Research and Therapeutic Potential
Given its role in critical cellular processes and disease progression, STAMBPL1 is a potential target for therapeutic interventions. Inhibitors or modulators of STAMBPL1 activity could be developed to treat cancers and other diseases associated with its dysregulation.
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