ST3GAL5 Human

ST3 Beta-Galactoside Alpha-2,3-Sialyltransferase 5 Human Recombinant

Cat. No.

BT13763

Source

Escherichia Coli.

Synonyms

ST3 Beta-Galactoside Alpha-2,3-Sialyltransferase 5, SIAT9, Sialyltransferase 9 (CMP-NeuAc:Lactosylceramide Alpha-2,3-Sialyltransferase; GM3 Synthase), Ganglioside GM3 Synthase, ST3GalV, CMP-NeuAc:Lactosylceramide Alpha-2,3-Sialyltransferase, ST3Gal V, EC 2.4.99.9, SATI, SIATGM3S, Alpha 2,3-Sialyltransferase V, Lactosylceramide Alpha-2,3-Sialyltransferase, Sialyltransferase 9, ST3GALV, GM3 Synthase.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

ST3GAL5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 359 amino acids (83-418a.a) and having a molecular mass of 41kDa. ST3GAL5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

ST3 Beta-Galactoside Alpha-2,3-Sialyltransferase 5, also known as ST3GAL5, is a member of the glycosyltransferase family 29 and is localized to the Golgi apparatus. This enzyme plays a crucial role in various cellular processes such as cell differentiation, proliferation, maintaining fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. ST3GAL5, a type II membrane protein, catalyzes the formation of GM3 using lactosylceramide as a substrate. Mutations in the ST3GAL5 gene have been linked to Amish infantile epilepsy syndrome. Several transcript variants encoding different isoforms of this gene have been identified.

Description

Recombinant human ST3GAL5, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 359 amino acids (83-418a.a). This protein has a molecular weight of 41 kDa and is fused to a 23 amino acid His-tag at its N-terminus. Purification is achieved using proprietary chromatographic techniques.

Physical Appearance

Clear, sterile-filtered solution.

Formulation

The ST3GAL5 protein solution is provided at a concentration of 1 mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4M Urea.

Stability

For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein like HSA or BSA (0.1%) is advisable. Repeated freeze-thaw cycles should be avoided.

Purity

The purity of the protein is greater than 85.0% as determined by SDS-PAGE analysis.

Synonyms

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSLKLNYTT EECDMKKMHY VDPDHVKRAQ KYAQQVLQKE CRPKFAKTSM ALLFEHRYSV DLLPFVQKAP KDSEAESKYD PPFGFRKFSS KVQTLLELLP EHDLPEHLKA KTCRRCVVIG SGGILHGLEL GHTLNQFDVV IRLNSAPVEG YSEHVGNKTT IRMTYPEGAP LSDLEYYSND LFVAVLFKSV DFNWLQAMVK KETLPFWVRL FFWKQVAEKI PLQPKHFRIL NPVIIKETAF DILQYSEPQS RFWGRDKNVP TIGVIAVVLA THLCDEVSLA GFGYDLNQPR TPLHYFDSQC MAAMNFQTMH NVTTETKFLL KLVKEGVVKD LSGGIDREF

Product Science Overview

Structure and Function

ST3GAL5 is a type II membrane protein localized in the Golgi apparatus. It belongs to the glycosyltransferase family 29 and catalyzes the transfer of sialic acid from CMP-sialic acid to the terminal galactose residues of lactosylceramide. This reaction is the first step in the synthesis of complex gangliosides, which are essential components of neural tissue.

Biological Significance

Gangliosides, including GM3, are involved in various biological processes such as cell-cell communication, cell growth, and differentiation. They are particularly abundant in the nervous system and play a critical role in neural development and function. Mutations in the ST3GAL5 gene have been associated with disorders such as Amish infantile epilepsy syndrome, highlighting the enzyme’s importance in normal neurological function.

Recombinant Production

The recombinant form of ST3GAL5 is produced using genetic engineering techniques, where the ST3GAL5 gene is cloned and expressed in suitable host cells. This allows for the large-scale production of the enzyme for research and therapeutic purposes. Recombinant ST3GAL5 is used in studies to understand its role in glycosphingolipid metabolism and its potential implications in neurological diseases.

Research and Therapeutic Applications

Research on ST3GAL5 has provided insights into the mechanisms of glycosphingolipid biosynthesis and the pathogenesis of related diseases. Therapeutically, targeting the enzyme’s activity could offer potential treatments for disorders caused by its dysfunction. Additionally, recombinant ST3GAL5 can be used in the development of diagnostic tools and therapeutic agents for neurological conditions.

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ST3GAL5 Human

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