SSU72 Antibody
SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae), HSPC182, CTD phosphatase SSU72, Ssu72 RNA polymerase II CTD phosphatase homolog (yeast), PNAS-120, RNA polymerase II subunit A C-terminal domain phosphatase SSU72, EC 3.1.3.16.
Description
Product Specs
SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae), HSPC182, CTD phosphatase SSU72, Ssu72 RNA polymerase II CTD phosphatase homolog (yeast), PNAS-120, RNA polymerase II subunit A C-terminal domain phosphatase SSU72, EC 3.1.3.16.
SSU72 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT66D10AT.
Anti-human SSU72 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human SSU72 amino acids 1-194 purified from E. coli.
Mouse IgG2b heavy chain and κ light chain.
Product Science Overview
SSU72 RNA Polymerase II CTD Phosphatase is a crucial enzyme involved in the regulation of gene expression. It specifically targets the C-terminal domain (CTD) of RNA Polymerase II, a key player in the transcription process. This enzyme is highly conserved across species, including humans and mice, making it a significant focus of research in molecular biology.
SSU72 is a protein phosphatase that catalyzes the dephosphorylation of the CTD of RNA Polymerase II . The CTD consists of multiple heptapeptide repeats, which are subject to phosphorylation and dephosphorylation cycles during transcription. These modifications are essential for the transition between different stages of transcription, including initiation, elongation, and termination.
The primary function of SSU72 is to remove phosphate groups from the serine residues in the CTD heptapeptide repeats. This dephosphorylation is crucial for the recycling of RNA Polymerase II and the regulation of gene expression . SSU72 also plays a role in RNA processing and termination, as well as pre-mRNA polyadenylation through its interaction with SYMPK .
The activity of SSU72 is tightly regulated and essential for proper transcriptional control. During the transcription cycle, RNA Polymerase II undergoes dynamic changes in its phosphorylation status. SSU72 is involved in the early stages of transcription, particularly during the formation of the preinitiation complex (PIC) and early elongation complexes . It has been shown that SSU72 is physically associated with these early transcription complexes and ceases to function on complexes containing RNA longer than 28 nucleotides .
Research on SSU72 has provided valuable insights into its role in transcription regulation. Studies have demonstrated that SSU72 is essential for the proper functioning of RNA Polymerase II and the regulation of gene expression . Additionally, SSU72 has been implicated in various cellular processes, including mRNA polyadenylation and RNA processing .
The mouse anti-human SSU72 antibody is a valuable tool for studying the function and regulation of SSU72 in different biological contexts. This antibody specifically targets the human SSU72 protein, allowing researchers to investigate its role in transcription and other cellular processes. It is commonly used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect and analyze SSU72 in various samples.
