SPRY4 Human

Sprouty Homolog 4 Human Recombinant
Cat. No.
BT20311
Source
Escherichia Coli.
Synonyms
Protein sprouty homolog 4, SPRY4, Sprouty Homolog 4, Spry-4, Protein sprouty homolog 4 isoform 2, Sprouty homolog 4 (Drosophila).
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SPRY4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 322 amino acids (1-299) and having a molecular mass of 34.9 kDa.
SPRY4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Sprouty Homolog 4 (SPRY4) is a negative regulator of the receptor-activated mitogen-activated protein kinase (MAPK) signaling pathway, acting at a point upstream of RAS activation. Among its various functions, SPRY4 protein is known to inhibit the EGFR-mediated MAPK signaling pathway and disrupt the formation of GTP-Ras.
Description
Recombinant human SPRY4, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 322 amino acids (residues 1-299) with a molecular weight of 34.9 kDa. It includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Sterile, colorless, and filtered solution.
Formulation
The SPRY4 solution is supplied at a concentration of 1 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4 M Urea.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 85.0% as assessed by SDS-PAGE analysis.
Synonyms
Protein sprouty homolog 4, SPRY4, Sprouty Homolog 4, Spry-4, Protein sprouty homolog 4 isoform 2, Sprouty homolog 4 (Drosophila).
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEPPIPQ SAPLTPNSVM VQPLLDSRMS HSRLQHPLTI LPIDQVKTSH VENDYIDNPS LALTTGPKRT RGGAPELAPT PARCDQDVTH HWISFSGRPS SVSSSSSTSS DQRLLDHMAP PPVADQASPR AVRIQPKVVH CQPLDLKGPA VPPELDKHFL LCEACGKCKC KECASPRTLP SCWVCNQECL CSAQTLVNYG TCMCLVQGIF YHCTNEDDEG SCADHPCSCS RSNCCARWSF MGALSVVLPC LLCYLPATGC VKLAQRGYDR LRRPGCRCKH TNSVICKAAS GDAKTSRPDK PF.

Product Science Overview

Discovery and Evolution

Sprouty proteins were first discovered in Drosophila melanogaster (fruit fly) as inhibitors of fibroblast growth factor (FGF)-mediated tracheal branching . The human homologs of Sprouty, including SPRY4, were identified later. SPRY4 is located on chromosome 5q31.3 in humans .

Function and Mechanism

SPRY4 functions as an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway . It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS . By doing so, SPRY4 suppresses the epidermal growth factor receptor (EGFR)-transduced MAPK signaling pathway . This inhibition is crucial for regulating cellular responses to growth signals and maintaining cellular homeostasis.

Biological Significance

The MAPK/ERK signaling pathway, regulated by SPRY4, is one of the most widespread regulatory mechanisms in eukaryotic cell biology . Dysregulation of this pathway can lead to various diseases, including cancer. Therefore, SPRY4’s role as a negative regulator is vital for preventing uncontrolled cell proliferation and tumorigenesis.

Research and Applications

Human recombinant SPRY4 is used in research to study its inhibitory effects on MAPK signaling and its potential therapeutic applications. Understanding SPRY4’s function and regulation can provide insights into developing targeted therapies for diseases associated with aberrant RTK signaling.

Interactions

SPRY4 has been shown to interact with various proteins, including TESK1 . These interactions further modulate its inhibitory effects on signaling pathways, highlighting the complexity of its regulatory mechanisms.

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