SPRR1B Human

Small Proline-Rich Protein 1B Human Recombinant
Cat. No.
BT20199
Source
Escherichia Coli.
Synonyms
Cornifin-B, 14.9 kDa pancornulin, Small proline-rich protein IB, SPR-IB, SPRR1B, SPRR1, GADD33, CORNIFIN.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SPRR1B Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 2-89) containing 97 amino acids including a 9 a.a N-terminal His tag. The total molecular mass is 10.9kDa (calculated).

Product Specs

Introduction
Small Proline-Rich Protein 1B (SPRR1B) is a protein found in keratinocytes. Initially found in the cell's cytosol, it eventually binds to membrane proteins through a process called transglutaminase. This binding results in the formation of a rigid envelope. SPRR1B acts as both a contributor and receiver of amine groups during the transglutaminase-mediated cross-linking process. The protein is expressed during the development of squamous tissues, such as the epidermis, esophagus, tongue, and trachea.
Description
Recombinantly produced in E. coli, SPRR1B Human is a single, non-glycosylated polypeptide chain comprising 97 amino acids (a.a 2-89), including a 9 a.a N-terminal His tag. This results in a calculated molecular mass of 10.9kDa.
Physical Appearance
White powder, lyophilized (freeze-dried) and filtered.
Formulation
SPRR1B is filtered (0.4 µm) and lyophilized from a 0.5mg/ml solution containing 0.05M phosphate buffer and 0.075M NaCl, at pH 7.4.
Solubility
To prepare a working stock solution, it is recommended to add deionized water to achieve a concentration of approximately 0.5mg/ml. Allow the lyophilized pellet to dissolve completely. Please note that SPRR1B is not sterile. It's crucial to filter the product through an appropriate sterile filter before introducing it into cell cultures.
Stability
The lyophilized protein should be stored at -20°C. After reconstitution, aliquot the product to minimize repeated freeze-thaw cycles. Reconstituted protein can be stored at 4°C for a limited period; it remains stable for up to two weeks at this temperature.
Purity
Purity levels exceed 95.0%, as determined by SDS-PAGE analysis.
Synonyms
Cornifin-B, 14.9 kDa pancornulin, Small proline-rich protein IB, SPR-IB, SPRR1B, SPRR1, GADD33, CORNIFIN.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHAS SQQQKQPCTP PPQLQQQQVK QPCQPPPQEP CIPKTKEPCH PKVPEPCHPK VPEPCQPKVP EPCHPKVPEP CPSIVTPAPA QQKTKQK.

Product Science Overview

Structure and Composition

SPRR1B is characterized by its high proline content and the presence of several tandem amino acid repeats. The protein is initially found in the cytosol of keratinocytes but eventually becomes cross-linked to membrane proteins by the enzyme transglutaminase. This cross-linking results in the formation of an insoluble layer beneath the plasma membrane, contributing to the structural integrity of the skin barrier .

Biological Functions

SPRR1B serves multiple functions, including:

  • Structural Role: It acts as a substrate for transglutaminase, facilitating the formation of the cornified envelope, which is essential for the barrier function of the skin .
  • Antimicrobial Activity: Recent studies have shown that SPRR1B, along with other SPRR proteins, exhibits bactericidal activity. These proteins can disrupt bacterial membranes, thereby defending the skin against microbial infections .
Regulation and Expression

The expression of SPRR1B is regulated by various factors, including exposure to bacterial components such as lipopolysaccharides (LPS). In response to LPS, SPRR1B expression is upregulated in human sebocytes, enhancing the skin’s antimicrobial defense mechanisms .

Clinical Relevance

Alterations in SPRR1B expression have been associated with several skin conditions and diseases. For instance, increased expression of SPRR1B has been observed in conditions like cervical intraepithelial neoplasia and mixed lacrimal gland cancer . Understanding the regulation and function of SPRR1B can provide insights into the pathogenesis of these diseases and potential therapeutic targets.

Research and Applications

Recombinant SPRR1B is used in various research applications to study its role in skin biology and its potential therapeutic uses. The recombinant form allows for detailed investigation of its structure, function, and interactions with other proteins.

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