SPON1 Human

Recombinant Human Spondin-1
Cat. No.
BT19289
Source

HEK293 Cells.

Synonyms

F-Spondin, VSGP

Appearance
Sterile filtered colorless solution.
Purity

Greater than 90% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SPON1 Human Recombinant produced in HEK293 cells is a single, glycosylated, polypeptide chain (29-807 a.a) containing a total of 785 amino acids, having a molecular mass of 88.9 kDa.
SPON1 is fused to a 6 amino acid His-tag at C-terminus,and is purified by proprietary chromatographic techniques.

Product Specs

Introduction
SPON1, a cell adhesion protein, plays a crucial role in regulating protein binding, processing, and the breakdown of amyloid precursor protein. Primarily located in the extracellular space, SPON1 co-localizes with collagen-rich extracellular matrix. Notably, it stimulates the attachment of spinal cord and sensory neuron cells, further promoting neurite outgrowth in laboratory settings.
Description
Recombinant human SPON1, produced in HEK293 cells, is a single, glycosylated polypeptide chain with a sequence spanning from amino acid 29 to 807. This chain comprises 785 amino acids, resulting in a molecular weight of 88.9 kDa. A 6-amino acid His-tag is fused to the C-terminus of SPON1. The protein is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has undergone sterile filtration.
Formulation
The SPON1 protein solution has a concentration of 0.25 mg/ml and is prepared in a phosphate buffer saline (pH 7.4) solution containing 30% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Repeated freezing and thawing should be avoided.
Purity
The purity of the protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms

F-Spondin, VSGP

Source

HEK293 Cells.

Amino Acid Sequence

FSDETLDKVP KSEGYCSRIL RAQGTRREGY TEFSLRVEGD PDFYKPGTSY RVTLSAAPPS YFRGFTLIAL RENREGDKEE DHAGTFQIID EEETQFMSNC PVAVTESTPR RRTRIQVFWI APPAGTGCVI LKASIVQKRI IYFQDEGSLT KKLCEQDSTF DGVTDKPILD CCACGTAKYR LTFYGNWSEK THPKDYPRRA NHWSAIIGGS HSKNYVLWEY GGYASEGVKQ VAELGSPVKM EEEIRQQSDE VLTVIKAKAQ WPAWQPLNVR AAPSAEFSVD RTRHLMSFLT MMGPSPDWNV GLSAEDLCTK ECGWVQKVVQ DLIPWDAGTD SGVTYESPNK PTIPQEKIRP LTSLDHPQSP FYDPEGGSIT QVARVVIERI ARKGEQCNIV PDNVDDIVAD LAPEEKDEDD TPETCIYSNW SPWSACSSST CDKGKRMRQR MLKAQLDLSV PCPDTQDFQP CMGPGCSDED GSTCTMSEWI TWSPCSISCG MGMRSRERYV KQFPEDGSVC TLPTEETEKC TVNEECSPSS CLMTEWGEWD ECSATCGMGM KKRHRMIKMN PADGSMCKAE TSQAEKCMMP ECHTIPCLLS PWSEWSDCSV TCGKGMRTRQ RMLKSLAELG DCNEDLEQVE KCMLPECPID CELTEWSQWS ECNKSCGKGH VIRTRMIQME PQFGGAPCPE TVQRKKCRIR KCLRNPSIQK LRWREARESR RSEQLKEESE GEQFPGCRMR PWTAWSECTK LCGGGIQERY MTVKKRFKSS QFTSCKDKKE IRACNVHPC-HHHHHH

 

Product Science Overview

Structure and Function

R-Spondin 1 is a secreted activator protein characterized by two cysteine-rich, furin-like domains and one thrombospondin type 1 domain . These domains are necessary and sufficient for potentiating Wnt signaling, which is vital for stem cell and organoid culture . The protein induces rapid onset of crypt cell proliferation and enhances intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects .

Production and Purification

Recombinant Human Spondin-1 is typically produced using Chinese Hamster Ovary (CHO) cells . The protein is purified to a high degree, with a purity of over 90% as determined by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining . The endotoxin level is kept below 0.10 EU per 1 μg of the protein by the LAL method .

Applications

R-Spondin 1 is widely used in cell culture, differentiation studies, and functional assays . It enhances BMP-2-mediated differentiation of MC3T3-E1 cells, with an expected ED50 of 1.0-3.0 μg/mL . The protein is also used in research focused on intestinal epithelial healing and protection against chemotherapy-induced damage .

Storage and Stability

Recombinant Human Spondin-1 is typically shipped at ambient temperature and should be stored at -20 to -70 °C to maintain its stability . It is recommended to use a manual defrost freezer and avoid repeated freeze-thaw cycles to preserve the protein’s integrity .

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THE BIOTEK
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