SPOCK3 Mouse

Sparc/Osteonectin 3 Mouse Recombinant
Cat. No.
BT19939
Source
Sf9, Baculovirus cells.
Synonyms
Testican-3, SPARC/osteonectin, CWCV, Kazal-like domains proteoglycan 3, Spock3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SPOCK3 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 423 amino acids (22-436 a.a.) and having a molecular mass of 47.9kDa (Migrates at 40-57kDa on SDS-PAGE under reducing conditions).
SPOCK3 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Testican-3, also known as Spock3, is a heparan sulfate proteoglycan found in the nervous system. Part of the BM-40/SPARC/osteonectin family, its exact role in brain development remains unclear. Spock3 is known to inhibit the processing of pro-matrix metalloproteinase 2 (MMP-2) by MT1-MMP and MT3-MMP. Its presence is primarily observed during the developmental stage of the brain.
Description
This product consists of recombinant SPOCK3 protein derived from mice. Produced in Sf9 Baculovirus cells, it exists as a single, glycosylated polypeptide chain with a molecular weight of 47.9kDa. The protein sequence encompasses amino acids 22-436 and includes an 8 amino acid His tag at the C-terminus. Purification is achieved using proprietary chromatographic techniques. On SDS-PAGE under reducing conditions, the protein migrates between 40-57kDa.
Physical Appearance
A clear and colorless solution, sterilized by filtration.
Formulation
The SPOCK3 protein solution has a concentration of 0.25mg/ml and is supplied in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
Purity of the SPOCK3 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Testican-3, SPARC/osteonectin, CWCV, Kazal-like domains proteoglycan 3, Spock3.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
AAAAVAVAGG RSDGGNFLDE KQWLTTISQY DKEVGQWNKF RDEVEDDYFR TWNPGKPFDQ ALDPAKDPCL KTKCSRHKVC ITQDAQTALC ISHRRLTHSM KEVGGSHKQW RGLPSSTCKP CPIAYASPVC GSDGHSYSSQ CKLEYQACVL GKQISIKCEG RCPCPSDKSM NIGRNVKRAC
SDLEFREVAN RLRDWFKALH ESGSQNKKTK ALLRPERSRF DTSILPICKD SLGWMFNRLD TNYDLLLDQS ELGSIYLDKN EQCTKAFFNS CDTYKDSLIS NNEWCYCFQR QQDPPCHTEL SNIQKRQGIK KLLGQYIPLC DEDGYYKPTQ CHGSVGQCWC VDRYGNEVVG SRINGVADCA
IDFEISGDFA SGDFREWTDD EGEEDDIMND KDDIEDDDED EGDDDDDGDV HDGYILEHHH HHH

Product Science Overview

Introduction

SPARC (Secreted Protein Acidic and Rich in Cysteine), also known as Osteonectin or BM-40, is a matricellular protein that plays a crucial role in bone biology. It is one of the most abundant non-collagenous proteins in bone and is involved in various cellular processes, including cell proliferation, differentiation, and matrix assembly .

Structure and Domains

SPARC consists of three distinct domains:

  1. EF-hand domain: This domain is involved in calcium binding.
  2. Follistatin-like domain: This domain has unique properties and independent activity.
  3. Kazal-like domain: This domain also has unique properties and independent activity .
Function and Mechanism

SPARC is known for its regulatory role in extracellular matrix (ECM) mineralization, which is a key step in bone repair and remodeling. It affects the differentiation, maturation, and mineralization of osteoblasts, the cells responsible for bone formation . SPARC has a high affinity for type 1 collagen and hydroxylapatite (HA), which are essential components of the bone matrix .

Role in Osteoblasts

Studies have shown that SPARC bidirectionally regulates osteoblast mineralization in a dose-dependent manner. It influences the expression of various genes and proteins involved in bone formation, such as bone sialoprotein (BSP), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) . Additionally, SPARC interacts with collagen and activates the P38 pathway, which is crucial for osteoblast function .

Recombinant SPARC/Osteonectin 3 in Research

Recombinant SPARC/Osteonectin 3 is used in research to study its effects on bone biology and its potential therapeutic applications. By using recombinant proteins, researchers can investigate the specific roles and mechanisms of SPARC in various cellular processes and disease models .

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