SPI1 Human

Spi-1 Proto-Oncogene Human Recombinant
Cat. No.
BT19782
Source
Escherichia Coli.
Synonyms
Transcription factor PU.1, 31 kDa-transforming protein, Transcription factor PU.1 isoform 1, SPI1, OF, PU.1, SFPI1, SPI-1, SPI-A.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SPI1 Human Recombinant produced in E. coli is a single polypeptide chain containing 294 amino acids (1-271) and having a molecular mass of 33.6kDa.
SPI1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The SPI1 gene encodes an ETS-domain transcription factor known as Spi-1 Proto-Oncogene. This factor plays a crucial role in activating gene expression during the development of myeloid and B lymphoid cells. SPI1 binds to a purine-rich DNA sequence called the PU-box, typically found near the promoters of its target genes, thereby regulating their expression. Additionally, SPI1 can bind to RNA and influence pre-mRNA splicing, including the alternative splicing of specific target genes.
Description
Recombinant human SPI1, expressed in E. coli, is a single polypeptide chain with a molecular weight of 33.6 kDa. It comprises 294 amino acids, with residues 1-271 corresponding to the SPI1 protein. A 23 amino acid His-tag is fused to the N-terminus to facilitate purification, which is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized through filtration.
Formulation
The SPI1 solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the SPI1 solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein such as HSA or BSA (0.1%) is advisable. It's important to avoid repeated cycles of freezing and thawing.
Purity
The purity of SPI1 is determined by SDS-PAGE analysis to be greater than 85.0%.
Synonyms
Transcription factor PU.1, 31 kDa-transforming protein, Transcription factor PU.1 isoform 1, SPI1, OF, PU.1, SFPI1, SPI-1, SPI-A.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMLQACKM EGFPLVPPQP SEDLVPYDTD LYQRQTHEYY PYLSSDGESH SDHYWDFHPH HVHSEFESFA ENNFTELQSV QPPQLQQLYR HMELEQMHVL DTPMVPPHPS LGHQVSYLPR MCLQYPSLSP AQPSSDEEEG ERQSPPLEVS DGEADGLEPG PGLLPGETGS KKKIRLYQFL LDLLRSGDMK DSIWWVDKDK GTFQFSSKHK EALAHRWGIQ KGNRKKMTYQ KMARALRNYG KTGEVKKVKK KLTYQFSGEV LGRGGLAERR HPPH.

Product Science Overview

Introduction

The Spi-1 proto-oncogene, also known as SPI1 or PU.1, is a critical transcription factor involved in the regulation of gene expression during the development of myeloid and B-lymphoid cells. This gene plays a pivotal role in the immune system, influencing the differentiation and function of various immune cells.

Gene and Protein Structure

SPI1 encodes a protein that belongs to the ETS-domain transcription factor family. The ETS domain is a conserved DNA-binding domain that allows the protein to bind to specific DNA sequences and regulate the transcription of target genes. The SPI1 protein binds to a purine-rich sequence known as the PU-box, which is located near the promoters of its target genes .

Function and Mechanism

The primary function of SPI1 is to activate gene expression during the development of myeloid and B-lymphoid cells. It achieves this by binding to the PU-box and regulating the transcription of genes involved in cell differentiation and proliferation. Additionally, SPI1 can bind RNA and modulate pre-mRNA splicing, further influencing gene expression .

Role in Immune System

SPI1 is essential for the development and function of the immune system. It regulates the differentiation of myeloid cells, such as macrophages and neutrophils, as well as B-lymphocytes. By controlling the expression of genes involved in immune cell development, SPI1 ensures the proper functioning of the immune response .

Clinical Significance

Mutations or dysregulation of SPI1 can lead to various hematological disorders and cancers. For instance, overexpression of SPI1 has been associated with certain types of leukemia. As a result, SPI1 is considered a potential target for therapeutic interventions in hematological malignancies .

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