SPECC1 Human

Sperm Antigen With Calponin Homology And Coiled-Coil 1 Human Recombinant
Cat. No.
BT19610
Source
Escherichia Coli.
Synonyms
CYTSB, HCMOGT-1, HCMOGT1, NSP, Cytospin-B, Nuclear structure protein 5, NSP5, Sperm antigen HCMOGT-1, Sperm antigen with calponin homology and coiled-coil domains 1, SPECC1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SPECC1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 327 amino acids (458-761a.a) and having a molecular mass of 37.7kDa.
SPECC1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Belonging to the cytospin-A family, Sperm Antigen With Calponin Homology And Coiled-Coil 1 (SPECC1) plays a crucial role in cytokinesis and spindle organization. This protein contributes to actin cytoskeleton organization and microtubule stabilization, making it essential for proper cell adhesion and migration.
Description
SPECC1 Human Recombinant, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 327 amino acids (458-761a.a). With a molecular mass of 37.7kDa, it features a 23 amino acid His-tag fused at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear and sterile solution.
Formulation
The SPECC1 protein solution (0.25mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 10% glycerol, 1mM DTT, and 0.1M NaCl.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein like 0.1% HSA or BSA is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of SPECC1 is determined to be greater than 80% using SDS-PAGE analysis.
Synonyms
CYTSB, HCMOGT-1, HCMOGT1, NSP, Cytospin-B, Nuclear structure protein 5, NSP5, Sperm antigen HCMOGT-1, Sperm antigen with calponin homology and coiled-coil domains 1, SPECC1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSTQEGKII ELEQKCTGIL EQGRFEREKL LNIQQQLTCS LRKVEEENQG ALEMIKRLKE ENEKLNEFLE LERHNNNMMA KTLEECRVTL EGLKMENGSL KSHLQGEKQK ATEASAVEQT AESCEVQEML KVARAEKDLL ELSCNELRQE LLKANGEIKH VSSLLAKVEK DYSYLKEICD HQAEQLSRTS LKLQEKASES DAEIKDMKET IFELEDQVEQ HRAVKLHNNQ LISELESSVI KLEEQKSDLE RQLKTLTKQM KEETEEWRRF QADLQTAVVV ANDIKCEAQQ ELRTVKRKLL EEEEKNA.

Product Science Overview

Introduction

Sperm Antigen With Calponin Homology And Coiled-Coil 1 (SPECC1) is a protein encoded by the SPECC1 gene. This protein is known for its role in the stability of microtubules and the reorganization of the actin cytoskeleton, which are crucial for cell adhesion and migration . SPECC1 is also referred to as HCMOGT1 and NSP in various studies .

Gene Structure and Expression

The SPECC1 gene is located on chromosome 17p11.2 and contains 17 exons spanning at least 227 kb . The gene encodes multiple splice variants, which are expressed in various tissues, including the testis, spleen, and some cancer cell lines . The protein contains an ATPase domain similar to that of SMC family members and a coiled-coil domain commonly seen in motor or structural proteins .

Function and Mechanism

SPECC1 plays a significant role in microtubule stability and actin cytoskeleton reorganization. It colocalizes with acetylated alpha-tubulin at microtubules, including mitotic spindles during cell division and in gap junctions involved in cell adhesion . The protein interacts with actin via its C-terminal domain and is involved in spindle orientation and cell polarity, which are essential for cell migration .

Clinical Significance

Mutations in the SPECC1 gene have been associated with various diseases, including geographic tongue and atrophic glossitis . Additionally, SPECC1 has been identified as a fusion partner to the platelet-derived growth factor receptor, beta gene (PDGFRB) in cases of juvenile myelomonocytic leukemia . The gene’s involvement in cell adhesion and migration makes it a potential target for cancer research and therapy.

Research and Applications

Research on SPECC1 has shown its high expression in the testis and its role in stabilizing microtubules containing acetylated actin . Overexpression of SPECC1 can distort the actin cytoskeleton and cell shape, leading to defects in cell adhesion and migration . These findings highlight the importance of SPECC1 in cellular processes and its potential as a therapeutic target.

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