SPAG7 Human
Sperm Associated Antigen 7 Human Recombinant
Cat. No.
BT19436
Source
Escherichia Coli.
Synonyms
ACRP, FSA-1, Sperm-associated antigen 7, SPAG7.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
SPAG7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 250 amino acids (1-227 a.a.) and having a molecular mass of 28kDa.
SPAG7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SPAG7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Sperm Associated Antigen 7 (SPAG7), containing a single R3H domain, is found in the nucleus of fetal brain cells.
Description
Recombinant human SPAG7, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 250 amino acids (residues 1-227) with a molecular weight of 28 kDa. This protein features a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile filtered solution.
Formulation
The SPAG7 protein solution (0.5 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 7.5), 0.15 M NaCl, 30% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
ACRP, FSA-1, Sperm-associated antigen 7, SPAG7.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMADLLGS ILSSMEKPPS LGDQETRRKA REQAARLKKL QEQEKQQKVE FRKRMEKEVS DFIQDSGQIK KKFQPMNKIE RSILHDVVEV AGLTSFSFGE DDDCRYVMIF KKEFAPSDEE LDSYRRGEEW DPQKAEEKRK LKELAQRQEE EAAQQGPVVV SPASDYKDKY SHLIGKGAAK DAAHMLQANK TYGCVPVANK RDTRSIEEAM NEIRAKKRLR QSGEELPPTS.
Product Science Overview
Expression and Localization
Recombinant Production
The recombinant form of SPAG7, often referred to as Human Recombinant SPAG7, is produced using Escherichia coli (E. coli) expression systems . The recombinant protein is typically fused with a His-tag at the N-terminus to facilitate purification. The production process involves several steps, including:
- Gene Cloning: The gene encoding SPAG7 is cloned into an expression vector.
- Transformation: The vector is introduced into E. coli cells.
- Expression: The E. coli cells are cultured under conditions that induce the expression of the SPAG7 protein.
- Purification: The recombinant protein is purified using chromatography techniques, often utilizing the His-tag for affinity purification .
Applications
Recombinant SPAG7 is used in various research applications, particularly in studies related to reproductive biology and fertility. It can also be used as an antigen in immunological studies to understand its role in the immune response.
Storage and Stability
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