SP100 Human

SP100 Recombinant Human
Cat. No.
BT19270
Source
Sf9 insect cells.
Synonyms
Nuclear dot-associated Sp100 protein, SP100 nuclear antigen, Speckled 100 kDa, SP100-HMG nuclear autoantigen, FLJ00340, Lysp100b.
Appearance
Purity
Greater than 80% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

Recombinant Sp100 antigen is a full-length cDNA coding having a molecular mass of 54,662 Dalton (pH 5.0).Shows a 79kDa band on SDS-PAGE. Sp100 protein is fused to a hexa-histidine purification tag.

Product Specs

Introduction
SP100 protein is a homodimeric protein involved in the regulation of gene expression. It interacts with HP1 family members of nonhistone chromosomal proteins, such as CBX5 and CBX3, through the PxVxL motif. Additionally, SP100 interacts with the Epstein-Barr virus protein EBNA-LP. The primary isoform, Sp100-A, has a predicted molecular weight of 54 kDa but displays atypical electrophoretic mobility, appearing as a 100 kDa protein.
Description
Recombinant Sp100 antigen consists of the full-length cDNA sequence, resulting in a protein with a molecular weight of 54,662 Daltons (at pH 5.0). It appears as a 79 kDa band on SDS-PAGE. This Sp100 protein is fused to a hexa-histidine tag for purification purposes.
Formulation
Sp100 is supplied in a buffer solution containing 16mM HEPES (pH 8.0), 400mM NaCl, and 20% glycerol.
Immunological Functions
1. Binds to human auto-antibodies of the IgG type. 2. Serves as a standard antigen in ELISA tests (e.g., checkerboard analysis for evaluating positive and negative sera panels) and immunodot assays.
Applications
Suitable for Western blot analysis using sera from patients with Primary Biliary Cholangitis (PBC) or a monoclonal antibody targeting the hexa-His-tag.
Coating Concentration
The recommended coating concentration ranges from 0.5 to 0.9 µg/ml, depending on the specific ELISA plate type and the coating buffer used. It is compatible with biotinylation and iodination processes.
Stability
For short-term storage (up to 2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Minimize repeated freeze-thaw cycles to preserve protein integrity.
Purity
Purity exceeds 80% as assessed by SDS-PAGE.
Synonyms
Nuclear dot-associated Sp100 protein, SP100 nuclear antigen, Speckled 100 kDa, SP100-HMG nuclear autoantigen, FLJ00340, Lysp100b.
Source
Sf9 insect cells.

Product Science Overview

Introduction

SP100 is a nuclear antigen protein encoded by the SP100 gene. It is a significant component of the PML (promyelocytic leukemia)-SP100 nuclear bodies, which are subnuclear organelles involved in various physiological processes, including cell growth, differentiation, and apoptosis . The recombinant form of this protein, SP100 Recombinant Human, is often used in research to study its functions and interactions.

Gene and Protein Structure

The SP100 gene is located on chromosome 2 and encodes a protein that is approximately 100 kDa in size . The protein consists of several domains, including a high-mobility group (HMG) domain, which is involved in DNA binding and chromatin organization . The recombinant version of SP100 is typically produced with a GST (glutathione S-transferase) tag at the N-terminal, which aids in its purification and detection .

Biological Functions

SP100 plays multiple roles in cellular processes:

  • Gene Regulation: SP100 functions as a transcriptional coactivator of ETS1 and ETS2, which are transcription factors involved in various cellular processes . It can also act as a corepressor under certain conditions, preventing ETS1 from binding to DNA .
  • Tumor Suppression: Together with PML, SP100 is considered a tumor suppressor. It is involved in the regulation of cell growth and apoptosis, making it a crucial player in cancer biology .
  • Immune Response: SP100 is implicated in the immune response, particularly in the context of viral infections. It interacts with viral proteins and may play a role in the cellular defense mechanisms against viruses like human cytomegalovirus and Epstein-Barr virus .
Recombinant Production

The recombinant SP100 protein is produced using an in vitro wheat germ expression system. This method ensures that the protein maintains its correct conformational folding, which is essential for its biological function . The protein is purified to a high degree, with a purity of over 80% as determined by SDS-PAGE and Coomassie blue staining .

Applications in Research

SP100 Recombinant Human is used in various research applications:

  • Western Blotting: To detect SP100 protein levels in different samples.
  • ELISA: For quantifying SP100 in various biological fluids.
  • Immunoaffinity Purification: To isolate SP100 from complex mixtures.
  • Protein Arrays: To study protein-protein interactions involving SP100 .

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