SOS1 Human
Sf9, Baculovirus cells.
SOS Ras/Rac Guanine Nucleotide Exchange Factor 1, Gingival Fibromatosis, Hereditary, 1, SOS-1, Son Of Sevenless Homolog 1 (Drosophila), Guanine Nucleotide Exchange Factor, Son Of Sevenless Homolog 1, GINGF, GGF1, GF1, HGF, NS4, Son of sevenless homolog 1.
Description
SOS1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 495 amino acids (564-1049a.a.) and having a molecular mass of 58.0kDa. (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
SOS1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
SOS1, alternatively known as Gingival Fibromatosis, functions as a guanine nucleotide exchange factor for the Ras and Rac proteins. This protein features several key domains, including the REM and CDC25 domains, which facilitate the catalytic activity of SOS1 toward Ras. Additionally, the histone fold DH/PH (Dbl homology & Pleckstrin homology) domains work together to promote GTP/GDP exchange for Rac. The activation of Ras proteins is triggered by GTP binding, while the subsequent hydrolysis of bound GTP to GDP and phosphate leads to the inactivation of signaling by these proteins. Guanine nucleotide exchange factors for RAS can catalyze GTP binding, and GTPase-activating proteins can enhance GTP hydrolysis.
Produced in Sf9 Baculovirus cells, SOS1 is a single, glycosylated polypeptide chain comprising 495 amino acids (564-1049a.a.) with a molecular mass of 58.0kDa. Notably, the molecular size on SDS-PAGE is expected to appear at approximately 50-70kDa. This SOS1 protein is expressed with a C-terminal 6 amino acid His tag and purified using proprietary chromatographic techniques.
The SOS1 protein solution is provided at a concentration of 0.25mg/ml and contains 20mM Tris-HCl (pH 7.5), 30% glycerol, 0.1M NaCl, 1mM DTT, and 0.2mM MgCl2.
SOS Ras/Rac Guanine Nucleotide Exchange Factor 1, Gingival Fibromatosis, Hereditary, 1, SOS-1, Son Of Sevenless Homolog 1 (Drosophila), Guanine Nucleotide Exchange Factor, Son Of Sevenless Homolog 1, GINGF, GGF1, GF1, HGF, NS4, Son of sevenless homolog 1.
Sf9, Baculovirus cells.
ADPEEQMRLP SADVYRFAEP DSEENIIFEE NMQPKAGIPI IKAGTVIKLI ERLTYHMYAD PNFVRTFLTT YRSFCKPQEL LSLIIERFEI PEPEPTEADR IAIENGDQPL SAELKRFRKE YIQPVQLRVL NVCRHWVEHH FYDFERDAYL LQRMEEFIGT VRGKAMKKWV ESITKIIQRK KIARDNGPGH NITFQSSPPT VEWHISRPGH IETFDLLTLH PIEIARQLTL LESDLYRAVQ PSELVGSVWT KEDKEINSPN LLKMIRHTTN LTLWFEKCIV ETENLEERVA VVSRIIEILQ VFQELNNFNG VLEVVSAMNS SPVYRLDHTF EQIPSRQKKI LEEAHELSED HYKKYLAKLR SINPPCVPFF GIYLTNILKT EEGNPEVLKR HGKELINFSK RRKVAEITGE IQQYQNQPYC LRVESDIKRF FENLNPMGNS MEKEFTDYLF NKSLEIEPRN PKPLPRFPKK YSYPLKSPGV RPSNPRPGTH HHHHH.
Product Science Overview
The SOS1 gene was initially identified in Drosophila melanogaster during studies on eye development. The sevenless gene in fruit flies is essential for the formation of the seventh photoreceptor cell in the compound eye. Mutations in the sevenless gene result in the absence of this photoreceptor, leading to the name “Son of Sevenless” for the downstream gene that interacts with it .
SOS1 acts as a guanine nucleotide exchange factor (GEF) for Ras proteins. Ras proteins function as molecular switches that cycle between an inactive GDP-bound state and an active GTP-bound state. SOS1 facilitates the exchange of GDP for GTP on Ras, thereby activating Ras and promoting downstream signaling pathways .
The activation of Ras by SOS1 is a critical step in various signaling cascades, including the MAP kinase pathway, which regulates cell proliferation, differentiation, and survival. SOS1 is also involved in the transduction of signals from growth factor receptors and adhesion receptors to the Ras proteins .
The SOS1 protein is composed of several important domains that contribute to its function:
- DH (Dbl Homology) domain: Involved in the activation of Rho family GTPases.
- PH (Pleckstrin Homology) domain: Binds to phosphoinositides and helps localize SOS1 to the plasma membrane.
- RasGEF domain: Responsible for the guanine nucleotide exchange activity on Ras.
- Histone domain: Involved in protein-protein interactions .
Mutations in the SOS1 gene have been associated with various genetic disorders. Dominant mutant alleles of SOS1 are known to cause Noonan syndrome, a developmental disorder characterized by short stature, congenital heart defects, and distinctive facial features. Additionally, SOS1 mutations have been linked to hereditary gingival fibromatosis type 1 .
