SORD Antibody
Sorbitol Dehydrogenase, Mouse Anti Human
Cat. No.
BT25728
Source
Synonyms
EC 1.1.1.14, SORD1, SORD, L-iditol 2-dehydrogenase, DHSO,
Sorbitol Dehydrogenase.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
SORD enzyme, a member of the zinc-containing alcohol dehydrogenase family, is widely found in the kidney and eye lens. This enzyme facilitates the zinc-dependent conversion of polyols like sorbitol and xylitol to their corresponding ketoses.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The solution contains 1mg/ml of the product in a buffer consisting of PBS at pH 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), keep at 4°C. For longer periods, store at -20°C. Avoid repeated freezing and thawing.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This antibody has undergone testing using ELISA and Western blot analysis to confirm its specificity and reactivity. However, optimal working dilutions should be determined empirically for different applications. A starting dilution of 1:1000 is recommended.
Synonyms
EC 1.1.1.14, SORD1, SORD, L-iditol 2-dehydrogenase, DHSO,
Sorbitol Dehydrogenase.
Purification Method
SORD antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT10F4AT.
Immunogen
Anti-human SORD mAb,
clone PAT10F4AT, is derived from hybridization of mouse F0 myeloma cells with
spleen cells from BALB/c mice immunized with a recombinant human SORD protein 1-357
amino acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
Mouse Anti-Human Sorbitol Dehydrogenase Antibody
Introduction
Mouse anti-human sorbitol dehydrogenase antibodies are monoclonal antibodies developed to specifically target and bind to human SDH. These antibodies are widely used in research and diagnostic applications to study the expression and function of SDH in various biological samples.
Production and Characteristics
The production of mouse anti-human SDH antibodies involves immunizing mice with human recombinant SDH protein. The spleen cells from these mice are then fused with myeloma cells to create hybridoma cells that produce the desired monoclonal antibodies. These antibodies are typically of the IgG1 subclass and are purified using protein A/G affinity chromatography .
Applications
Mouse anti-human SDH antibodies are used in several scientific applications, including:
- Western Blotting (WB): To detect and quantify SDH protein levels in tissue and cell lysates.
- Immunohistochemistry (IHC): To visualize the localization of SDH in tissue sections.
- Flow Cytometry: To analyze the expression of SDH on the cell surface or within cells.
- Immunoprecipitation (IP): To isolate and study SDH protein complexes .
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