SOD1 Antibody

Superoxide Dismutase-1, Mouse Anti Human
Cat. No.
BT25638
Source
Synonyms
Superoxide dismutase [Cu-Zn], EC 1.15.1.1, SOD1, SOD, ALS, ALS1, IPOA.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Human Cu/Zn Superoxide Dismutase (SOD1) is an enzyme that facilitates the conversion of superoxide anions to molecular oxygen and hydrogen peroxide using hydrogen. This process is crucial for cellular protection against excessive superoxide levels. SOD1, one of three isozymes responsible for neutralizing free superoxide radicals in the body, achieves this by binding copper and zinc ions. Essentially, the encoded protein neutralizes supercharged oxygen molecules, which can cause cellular damage if their levels are not regulated. Mutations in the SOD1 gene can lead to a familial form of amyotrophic lateral sclerosis.
Physical Appearance
The product is a sterile, colorless solution that has been filtered.
Formulation
The product is provided at a concentration of 1mg/ml and is formulated in a solution containing PBS at a pH of 7.4, 10% Glycerol, and 0.02% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), the product should be kept at 4°C. For longer storage durations, it is recommended to store the product at -20°C. Repeated freezing and thawing of the product should be avoided.
Stability / Shelf Life
The product has a shelf life of 12 months when stored at -20°C and 1 month when stored at 4°C.
Applications
This antibody has undergone rigorous testing in various applications, including ELISA, Western blot analysis, Flow cytometry, and ICC/IF, to ensure its specificity and reactivity. However, it's important to note that optimal working dilutions may vary depending on the specific application and should be determined through titration.
Synonyms
Superoxide dismutase [Cu-Zn], EC 1.15.1.1, SOD1, SOD, ALS, ALS1, IPOA.
Purification Method
SOD1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT6F1AT.
Immunogen
Anti-human SOD1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SOD1 protein 1-154 amino acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.

Product Science Overview

Introduction

Superoxide dismutase-1 (SOD1) is a critical enzyme that plays a vital role in protecting cells from oxidative stress by catalyzing the dismutation of superoxide radicals into oxygen and hydrogen peroxide. This enzyme is highly conserved across species, including humans and mice. The mouse anti-human SOD1 antibody is a monoclonal antibody specifically designed to target and bind to human SOD1, making it a valuable tool in research and diagnostic applications.

Structure and Function of SOD1

SOD1 is a metalloenzyme that typically contains copper and zinc ions at its active site. The enzyme exists as a homodimer, with each subunit consisting of approximately 153 amino acids. The primary function of SOD1 is to catalyze the conversion of superoxide radicals (O2•−) into molecular oxygen (O2) and hydrogen peroxide (H2O2), thereby mitigating the potential damage caused by reactive oxygen species (ROS) in cells.

Role in Disease

Mutations in the SOD1 gene are associated with familial amyotrophic lateral sclerosis (fALS), a neurodegenerative disorder characterized by the progressive loss of motor neurons. These mutations can lead to the misfolding and aggregation of SOD1, which exhibits prion-like properties and can propagate the disease . Studies have shown that both wild-type and mutant forms of human SOD1 can induce ALS-like symptoms in transgenic mouse models .

Mouse Anti-Human SOD1 Antibody

The mouse anti-human SOD1 antibody is a monoclonal antibody that specifically recognizes and binds to human SOD1. This antibody is produced by immunizing mice with human SOD1 protein, followed by the fusion of spleen cells from the immunized mice with myeloma cells to create hybridomas. These hybridomas are then screened for the production of antibodies that specifically target human SOD1.

Applications in Research

The mouse anti-human SOD1 antibody is widely used in various research applications, including:

  • Western Blotting: To detect and quantify SOD1 protein levels in different tissue samples.
  • Immunohistochemistry: To visualize the distribution and localization of SOD1 in tissue sections.
  • Enzyme-Linked Immunosorbent Assay (ELISA): To measure SOD1 concentrations in biological samples.
  • Immunoprecipitation: To isolate and study SOD1 protein complexes.

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Source
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