SNUPN Human

Snurportin 1 Human Recombinant
Cat. No.
BT18113
Source
Escherichia Coli.
Synonyms
KPNBL, RNUT1, Snurportin1, SPN1, RNA U transporter 1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SNUPN Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 380 amino acids (1-360 a.a.) and having a molecular mass of 43.3 kDa. The SNUPN is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
SNUPN, a member of the Snurportin family, functions as a nuclear import adaptor protein. It resides in both the cytoplasm and nucleus and possesses an N-terminal IBB domain along with a trimethylguanosine (m3G)-cap binding domain. SNUPN exhibits specific binding to the terminal 2,2,7-m3G-cap situated at the 5' end of U snRNPs. Its role involves facilitating the transport of U snRNPs into the nucleus through interaction with Importin β.
Description
Recombinant Human SNUPN, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 380 amino acids (specifically, residues 1-360) and exhibits a molecular weight of 43.3 kDa. A 20 amino acid His-Tag is fused to the N-terminus of SNUPN. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
The product appears as a sterile, colorless solution after filtration.
Formulation
The provided solution of Human SNUPN contains 20mM Tris (pH 8), 2mM DTT, 0.1M NaCl, and 10% glycerol.
Stability
For optimal storage, refrigerate the product at 4°C if the entire vial's contents will be utilized within 2-4 weeks. For extended storage, freeze the product at -20°C. Adding a carrier protein (either 0.1% HSA or BSA) is recommended for long-term storage. It's essential to avoid subjecting the product to multiple freeze-thaw cycles.
Purity
SDS-PAGE analysis indicates a purity exceeding 90.0%.
Synonyms
KPNBL, RNUT1, Snurportin1, SPN1, RNA U transporter 1.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MEELSQALAS SFSVSQDLNS TAAPHPRLSQ YKSKYSSLEQ SERRRRLLEL QKSKRLDYVN HARRLAEDDW TGMESEEENK KDDEEMDIDT VKKLPKHYAN QLMLSEWLID VPSDLGQEWI VVVCPVGKRA LIVASRGSTS AYTKSGYCVN RFSSLLPGGN RRNSTAKDYT ILDCIYNEVN QTYYVLDVMC WRGHPFYDCQ TDFRFYWMHS KLPEEEGLGE KTKLNPFKFV GLKNFPCTPE SLCDVLSMDF PFEVDGLLFY HKQTHYSPGS TPLVGWLRPY MVSDVLGVAV PAGPLTTKPD YAGHQLQQIM EHKKSQKEGM KEKLTHKASE NGHYELEHLS
TPKLKGSSHS PDHPGCLMEN.

Product Science Overview

Structure and Function

Snurportin 1 contains two key domains:

  1. N-terminal Importin Beta Binding (IBB) Domain: This domain is essential for the interaction with Importin beta, a protein that facilitates the transport of cargo proteins into the nucleus.
  2. Trimethylguanosine (m3G)-Cap Binding Domain: This domain specifically binds to the 2,2,7-trimethylguanosine cap at the 5’ end of U snRNPs .

The primary function of Snurportin 1 is to recognize and bind the m3G-cap structure of U snRNPs and mediate their transport into the nucleus through its association with Importin beta . This process is vital for the proper functioning of the spliceosome, a complex responsible for pre-mRNA splicing.

Recombinant Snurportin 1

Recombinant Snurportin 1 is produced using various expression systems, such as Escherichia coli (E. coli) and HEK-293 cells. The recombinant protein typically includes a tag, such as a His-tag or Myc-DYKDDDDK tag, to facilitate purification and detection .

  • E. coli Expression System: In this system, the recombinant Snurportin 1 is expressed as a single, non-glycosylated polypeptide chain containing 380 amino acids (1-360 a.a.) with a molecular mass of approximately 43.3 kDa. The protein is fused to a 20 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques .
  • HEK-293 Cells Expression System: In this system, the recombinant Snurportin 1 is expressed with a Myc-DYKDDDDK tag. The protein is produced with end-sequenced ORF clone and has a purity of over 80% as determined by SDS-PAGE and Coomassie blue staining .
Applications

Recombinant Snurportin 1 is used in various research applications, including:

  • Antibody Production: As a native antigen for optimized antibody production.
  • Positive Controls: In ELISA and other antibody assays.
  • Protein-Protein Interaction Studies: To study the interactions between Snurportin 1 and other proteins involved in nuclear import and spliceosome assembly .
Storage and Handling

Recombinant Snurportin 1 should be stored at -80°C for long-term storage. It is recommended to thaw the protein on ice, aliquot it into individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze-thaw cycles are recommended to maintain protein integrity .

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