SNTN Human

Sentan Cilia Apical Structure Protein Human Recombinant
Cat. No.
BT2043
Source
Escherichia Coli.
Synonyms
Sentan cilia apical structure protein, FLJ44379, S100AL, S100A1L, S100A-like protein, sentan, S100 calcium-binding protein A1-like.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SNTN Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 167 amino acids (1-147a.a.) and having a molecular mass of 18.6kDa.
The SNTN is purified by proprietary chromatographic techniques.

Product Specs

Introduction
SNTN, a member of the S-100 family, is exclusively found in the bridging structure connecting the cell membrane and peripheral singlet microtubules. This structure is uniquely present in the narrowed distal part of cilia. Studies have shown that sentan, when expressed externally, exhibits an affinity for membrane protrusions. Furthermore, a protein-lipid binding assay revealed that sentan binds to phosphatidylserine. These findings suggest that sentan is the primary molecular component at the ciliary tip responsible for linking the cell membrane and peripheral singlet microtubules. This connection contributes to the narrow and rigid nature of the distal cilia region, facilitating optimal airway clearance or ovum transport.
Description
Recombinant Human SNTN, with a 20 amino acid His tag attached at the N-terminus, is produced in E. coli. This single, non-glycosylated polypeptide chain comprises 167 amino acids (1-147a.a.) and has a molecular weight of 18.6 kDa. The purification of SNTN is carried out using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The SNTN solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, 50% glycerol, and 0.15M NaCl.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the product is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
Sentan cilia apical structure protein, FLJ44379, S100AL, S100A1L, S100A-like protein, sentan, S100 calcium-binding protein A1-like.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGGCMHSTQD KSLHLEGDPN PSAAPTSTCA PRKMPKRISI SKQLASVKAL RKCSDLEKAI ATTALIFRNS SDSDGKLEKA IAKDLLQTQF RNFAEGQETK PKYREILSEL DEHTENKLDF EDFMILLLSI TVMSDLLQNI RNVKIMK

Product Science Overview

Gene and Protein Information

The SNTN gene is located on chromosome 3 and is responsible for coding the Sentan protein . The protein is predicted to enable calcium ion binding activity and calcium-dependent protein binding activity . It is primarily located in the cilium, where it may act as a component of the linker structure that bridges the ciliary membrane and peripheral singlet microtubules .

Function and Importance

Sentan Cilia Apical Structure Protein is believed to be involved in maintaining the structural integrity of cilia. Cilia are critical for various physiological functions, including:

  • Motility: Cilia help in the movement of cells or the fluid surrounding them.
  • Sensory Functions: Cilia are involved in sensing environmental signals and transmitting them to the cell.
  • Signaling Pathways: Cilia play a role in various signaling pathways that regulate cell growth, differentiation, and development.

Defects in ciliary structure or function can lead to a group of disorders known as ciliopathies, which can affect multiple organ systems.

Recombinant Protein

Recombinant Sentan Cilia Apical Structure Protein is produced using recombinant DNA technology. This involves inserting the SNTN gene into a suitable expression system, such as bacteria or mammalian cells, to produce the protein in large quantities. The recombinant protein is then purified for use in research and therapeutic applications.

The recombinant human Sentan Cilia Apical Structure Protein is typically provided in a solution containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 50% glycerol, and 0.15M NaCl . This formulation helps maintain the stability and activity of the protein.

Research and Applications

Research on Sentan Cilia Apical Structure Protein is ongoing to better understand its role in ciliary function and its potential implications in ciliopathies. The recombinant protein is used in various experimental setups to study its biochemical properties, interactions with other proteins, and its role in cellular processes.

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