SNRPC Human, Sf9
Description
SNRPC Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 25,000 Dalton.
SNRPC is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
SNRPC is one of the specific protein constituents of the U1 snRNP particle. The U1 snRNP particle plays a pivotal role in recognizing the 5’ splice site of pre-mRNA during the splicing process . The interaction between SNRPC and the 5’ splice site is a critical step in the assembly of the spliceosome and the subsequent splicing of pre-mRNA .
The protein encoded by the SNRPC gene is involved in the initial recognition of the 5’ splice site for both constitutive and regulated alternative splicing . This interaction precedes the base-pairing between the pre-mRNA and the U1 small nuclear RNA (snRNA), highlighting the importance of SNRPC in the early stages of the splicing pathway .
The recombinant form of SNRPC, expressed in Sf9 cells, is produced using baculovirus expression systems. Sf9 cells, derived from the fall armyworm (Spodoptera frugiperda), are commonly used in the production of recombinant proteins due to their high expression levels and ability to perform post-translational modifications similar to those in higher eukaryotes .
The recombinant SNRPC protein is purified through a series of chromatographic techniques to ensure its purity and functionality. This recombinant form is used in various research applications, including studies on RNA splicing, protein-protein interactions, and the development of therapeutic interventions for diseases associated with splicing defects .
SNRPC is of particular interest in the study of autoimmune diseases. Patients with connective tissue diseases, such as systemic lupus erythematosus, often produce autoantibodies against snRNP particles, including SNRPC . These autoantibodies can interfere with the normal function of the spliceosome, leading to aberrant splicing and the production of defective mRNA transcripts.
Additionally, mutations or dysregulation of the SNRPC gene have been associated with various diseases, including lymphangiosarcoma . Understanding the role of SNRPC in these diseases can provide insights into their pathogenesis and potential therapeutic targets.
