SNRNP70 Human

Small Nuclear Ribonucleoprotein 70kDa (snRNP70), also known as U1 small nuclear ribonucleoprotein 70 kDa, is a protein encoded by the SNRNP70 gene in humans . This protein is a crucial component of the spliceosome, a complex responsible for the splicing of precursor mRNA molecules .

The snRNP70 protein associates with U1 spliceosomal RNA, forming the U1-snRNP complex, which is a core component of the spliceosome . The U1-snRNP is the most abundant ribonucleoprotein particle in the nucleus and consists of one small uridylate-rich RNA (U1 RNA) complexed with several proteins . These proteins include the unique 68/70 kDa, A, and C polypeptides, as well as seven Sm proteins (B/B’, D1, D2, D3, E, F, G) that form a core subparticle common to all U-snRNP complexes .

Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein, which lacks 66 internal amino acids outside the known epitope-containing areas . This recombinant protein is fused to a hexahistidine purification tag, facilitating its purification and use in various applications .

Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease (MCTD) and 30% of patients with Systemic Lupus Erythematosus (SLE) . Antibodies against the 68/70 kDa protein are known to have high clinical significance in MCTD patients . The use of single recombinant proteins as antigenic targets guarantees higher sensitivity and specificity in detecting these autoantibodies .

The recombinant snRNP70 protein is used in various immunological assays, including Western Blot and ELISA . It binds IgG-type human auto-antibodies and is suitable for labeling functional groups . The protein is supplied in a sterile filtered clear solution and is stable when stored at 4°C for short periods or frozen at -20°C for longer periods .