SNRNP25 Human

The U12-type spliceosome, which includes SNRNP25, is responsible for the removal of U12-type introns from pre-mRNA. This process is crucial for the proper maturation of mRNA, which in turn is essential for the accurate translation of genetic information into functional proteins . The major U2-type spliceosome removes the more common U2-type introns, while the U12-type spliceosome handles the less common U12-type introns .

Recombinant SNRNP25 refers to the protein that has been genetically engineered and produced in a laboratory setting, often using an expression system such as E. coli . This recombinant form is used in various research applications, including blocking assays and control experiments . The recombinant protein is typically purified and supplied in a liquid form, with a concentration of at least 5.0 mg/mL .

Recombinant SNRNP25 is valuable in research for several reasons:

• Blocking Assays: It can be used to block specific interactions in experiments, helping to validate the specificity of antibodies .
• Control Experiments: It serves as a control to ensure the accuracy and reliability of experimental results .

The recombinant SNRNP25 protein is usually stored in a buffer solution containing 1M urea/PBS at pH 7.4 and should be kept at -20°C to avoid freeze/thaw cycles . Proper storage is essential to maintain the protein’s stability and functionality.

The SNRNP25 gene is located on chromosome 16 and is also known by several aliases, including C16orf33 and Minus-99 protein . The gene’s protein product has a molecular weight of approximately 25 kDa and is involved in the minor pathway of mRNA splicing .

Mutations or dysregulation of the SNRNP25 gene may be associated with certain diseases, such as familial hemophagocytic lymphohistiocytosis . Understanding the role and function of SNRNP25 can provide insights into these conditions and potentially lead to the development of targeted therapies.