SNIP1 Human

Smad Nuclear Interacting Protein 1 Human Recombinant
Cat. No.
BT17829
Source
Escherichia Coli.
Synonyms

Smad nuclear-interacting protein 1, FHA domain-containing protein SNIP1, SNIP1, FLJ12553, dJ423B22.2, RP3-423B22.3.

Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SNIP1 Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 160 amino acids (258-396 a.a.) and having a molecular mass of 18.8kDa.
The SNIP1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
SNIP1, a smad nuclear interacting protein, possesses a forkhead-associated (FHA) domain and functions as a nuclear inhibitor of CBP/p300. This protein plays a crucial role in inhibiting the TGF-beta signal transduction pathway by suppressing transcriptional activation reliant on the co-activators CBP and p300. Notably, the N-terminal domain of SNIP1 is responsible for inhibiting NF-kappa B activity. This inhibition involves SNIP1 competing with the NF-kappa B subunit, RelA/p65, for binding to p300, a mechanism similar to how SNIP1 inhibits Smad signaling.
Description
SNIP1 Human Recombinant, fused with a 21 amino acid His tag at its N-terminus, is produced in E. coli. This single, non-glycosylated polypeptide chain comprises 160 amino acids (258-396 a.a.) and exhibits a molecular mass of 18.8 kDa. Purification of SNIP1 is achieved through proprietary chromatographic techniques.
Physical Appearance
A sterile, filtered solution that is colorless.
Formulation
The SNIP1 solution is provided at a concentration of 1 mg/ml and contains the following components: 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 20% glycerol, and 100mM NaCl.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. It is important to avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of the SNIP1 protein is greater than 90.0%, as determined by SDS-PAGE analysis.
Synonyms

Smad nuclear-interacting protein 1, FHA domain-containing protein SNIP1, SNIP1, FLJ12553, dJ423B22.2, RP3-423B22.3.

Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MRWRLYPFKN DEVLPVMYIH RQSAYLLGRH RRIADIPIDH PSCSKQHAVF QYRLVEYTRA DGTVGRRVKP YIIDLGSGNG TFLNNKRIEP QRYYELKEKD VLKFGFSSRE YVLLHESSDT SEIDRKDDED EEEEEEVSDS.

Product Science Overview

Introduction

Smad Nuclear Interacting Protein 1 (SNIP1) is a protein encoded by the SNIP1 gene in humans. This protein plays a crucial role in various cellular processes, including transcription regulation, cell proliferation, and immune response. The recombinant form of this protein is produced using recombinant DNA technology, which allows for the expression of the protein in a host organism, typically bacteria or yeast.

Structure and Function

SNIP1 contains a coiled-coil motif and a C-terminal forkhead-associated (FHA) domain . The coiled-coil motif is involved in protein-protein interactions, while the FHA domain is known for its role in recognizing phosphopeptides. These structural features enable SNIP1 to interact with various transcriptional coactivators and inhibitors, thereby modulating gene expression.

SNIP1 functions as a transcriptional coactivator that increases the activity of c-Myc, a well-known oncogene . Additionally, it inhibits the signaling pathways of transforming growth factor-beta (TGF-beta) and nuclear factor kappa-B (NF-kB), both of which are critical in regulating immune responses and inflammation .

Biological Significance

SNIP1 plays a significant role in several biological processes:

  1. Cell Proliferation and Cancer Progression: By regulating the stability of cyclin D1 mRNA, SNIP1 influences cell cycle progression and proliferation. This regulation is crucial in cancer biology, as dysregulated cell proliferation is a hallmark of cancer .

  2. Immune Regulation: SNIP1 inhibits NF-kB signaling by competing with RELA for binding to CREBBP/EP300. This inhibition is essential in controlling inflammatory responses and maintaining immune homeostasis .

  3. Cardiac Hypertrophy: Recent studies have shown that SNIP1 has protective effects against pressure overload-induced cardiac hypertrophy, fibrosis, and contractile dysfunction. It achieves this by inhibiting NF-kB signaling, which is activated during pathological cardiac hypertrophy .

Clinical Implications

Mutations in the SNIP1 gene are associated with several disorders, including psychomotor retardation, epilepsy, and craniofacial dysmorphism (PMRED) . Understanding the role of SNIP1 in these conditions can provide insights into potential therapeutic targets for treating these disorders.

Research and Therapeutic Potential

The recombinant form of SNIP1 is valuable in research and therapeutic applications. By studying the recombinant protein, researchers can gain a deeper understanding of its structure, function, and interactions. This knowledge can lead to the development of novel therapeutic strategies for diseases associated with SNIP1 dysregulation.

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