SNF8 Human

SNF8, ESCRT-II Complex Subunit Human Recombinant
Cat. No.
BT17746
Source
E.coli.
Synonyms
SNF8 ESCRT-II complex subunit homolog (S. cerevisiae), ESCRT-II complex subunit VPS22, ELL-associated protein of 30 kDa, EAP30 subunit of ELL complex, vacuolar-sorting protein SNF8, EAP30, VPS22, Dot3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SNF8 Human Recombinant produced in E. coli is a single polypeptide chain containing 282 amino acids (1-258) and having a molecular mass of 31.4 kDa.
SNF8 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
SNF8, a member of the SNF8 family of vacuolar sorting proteins, is found in both the nucleus and cytoplasm. It is a component of the endosomal sorting complex required for transport II (ESCRT-II), which is crucial for the formation of multivesicular bodies (MVBs) and the sorting of endosomal cargo proteins into these MVBs. The MVB pathway facilitates the degradation of transmembrane proteins by transferring them into the lysosome. Furthermore, the ESCRT-II complex plays a role in transcription regulation by contributing to the derepression of transcription by RNA polymerase II, potentially through its interaction with ELL.
Description
Recombinant human SNF8, produced in E. coli, is a single polypeptide chain consisting of 282 amino acids (1-258) with a molecular weight of 31.4 kDa. A 24 amino acid His-tag is fused to the N-terminus of SNF8, and it is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that is sterile and filtered.
Formulation
The SNF8 solution is provided at a concentration of 0.25 mg/ml and contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 2mM DTT, and 50% glycerol.
Stability
For optimal storage, keep the vial at 4°C if it will be used within 2-4 weeks. For extended storage, freeze the solution at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for long-term storage. It is essential to avoid repeated freezing and thawing cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
SNF8 ESCRT-II complex subunit homolog (S. cerevisiae), ESCRT-II complex subunit VPS22, ELL-associated protein of 30 kDa, EAP30 subunit of ELL complex, vacuolar-sorting protein SNF8, EAP30, VPS22, Dot3.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMHRRGV GAGAIAKKKL AEAKYKERGT VLAEDQLAQM SKQLDMFKTN LEEFASKHKQ EIRKNPEFRV QFQDMCATIG VDPLASGKGF WSEMLGVGDF YYELGVQIIE VCLALKHRNG GLITLEELHQ QVLKGRGKFA QDVSQDDLIR AIKKLKALGT  GFGIIPVGGT YLIQSVPAEL NMDHTVVLQL AEKNGYVTVS EIKASLKWET ERARQVLEHL LKEGLAWLDL QAPGEAHYWL PALFTDLYSQ EITAEEAREA LP

Product Science Overview

Introduction

SNF8 is a protein that plays a crucial role in the endosomal sorting complex required for transport II (ESCRT-II). This complex is essential for the regulation of various cellular processes, including the sorting and degradation of ubiquitinylated transmembrane proteins. The human recombinant form of SNF8 is used in various research applications to study its function and interactions within the cell.

Structure and Composition

SNF8 is a subunit of the ESCRT-II complex, which also includes VPS25 and VPS36 . The stability of the ESCRT-II complex is highly dependent on the presence of SNF8. The ESCRT-II complex is involved in the formation of multivesicular bodies (MVBs), which are essential for the delivery of transmembrane proteins to the lysosome for degradation .

Function

The primary function of SNF8 within the ESCRT-II complex is to facilitate the sorting of endosomal cargo proteins into MVBs. This process is crucial for the degradation of endocytosed proteins, such as the epidermal growth factor receptor (EGFR) and the chemokine receptor CXCR4 . Additionally, SNF8 is involved in the exosomal release of certain proteins, including SDCBP, CD63, and syndecan .

Interactions

SNF8 interacts with various proteins to carry out its functions. It is known to interact with the transcription elongation factor ELL, which helps overcome the repressive effects of ELL on RNA polymerase II activity . SNF8 also interacts with TSG101, another component of the ESCRT machinery . These interactions are essential for the proper functioning of the ESCRT-II complex and the regulation of protein sorting and degradation.

Research Applications

The human recombinant form of SNF8 is widely used in research to study its role in cellular processes. Researchers use recombinant SNF8 to investigate its interactions with other proteins, its involvement in the ESCRT-II complex, and its impact on cellular functions. Studies have shown that overexpression of SNF8 can enhance the activity of certain ion channels, such as TRPC6, and modulate transcriptional activity .

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