SNCA NACP112 Human
Description
A-Synuclein NACP112 Human Recombinant which is an alternatively spliced (103-129) form of a-Synuclein, produced in E.Coli is a single, non-glycosylated polypeptide chain of 112 amino acids having a molecular mass of 11.3kDa. The Recombinant Human a-Synuclein NACP112 is purified by proprietary chromatographic techniques.
Product Specs
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKEGYQDYEP EA.
Product Science Overview
Alpha Synuclein NACP112 is an alternatively spliced form of alpha-synuclein, consisting of 112 amino acids. It is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain with a molecular mass of approximately 11.3 kDa . The protein is highly heat-resistant and natively unfolded, primarily composed of random coils .
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are involved in the regulation of synaptic vesicle trafficking and neurotransmitter release. Alpha-synuclein, in particular, has been suggested to play a role in the pathogenesis of Parkinson’s disease and related neurodegenerative disorders . It is a major component of Lewy bodies, which are characteristic of Parkinson’s disease, and amyloid plaques found in Alzheimer’s disease .
The recombinant form of Alpha Synuclein NACP112 is produced by cloning the gene into an E. coli expression vector. The protein is then purified using conventional column chromatography techniques, taking advantage of its thermosolubility . The final product is a sterile-filtered, colorless solution with a purity greater than 95% as determined by SDS-PAGE .
Alpha Synuclein NACP112 is widely used in research to study the mechanisms underlying neurodegenerative diseases. It serves as a valuable tool for investigating the aggregation properties of alpha-synuclein and its interactions with other cellular components. Additionally, it is used in the development of potential therapeutic strategies aimed at preventing or reversing the aggregation of alpha-synuclein .
For optimal stability, Alpha Synuclein NACP112 should be stored at -20°C for long-term storage. It is recommended to add a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), to prevent multiple freeze-thaw cycles . When stored properly, the protein remains stable for extended periods, making it suitable for various experimental applications .
