SNAPC1 Human

Small Nuclear RNA Activating Complex, Polypeptide 1 Human Recombinant
Cat. No.
BT17673
Source
Escherichia Coli.
Synonyms
PTFgamma, SNAP43, snRNA-activating protein complex subunit 1, SNAPc subunit 1, Proximal sequence element-binding transcription factor subunit gamma, PSE-binding factor subunit gamma, PTF subunit gamma, Small nuclear RNA-activating complex polypeptide 1, SNAPc 43 kDa subunit, SNAPC1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SNAPC1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 391 amino acids (1-368 a.a) and having a molecular mass of 45.4kDa.
SNAPC1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Small Nuclear RNA Activating Complex, Polypeptide 1 (SNAPC1) is a component of the SNAPc complex, which is essential for the transcription of small nuclear RNA genes by both RNA polymerase II and III. SNAPC1 interacts with the proximal sequence element (PSE), a core promoter element found in these gene types that is distinct from the TATA box. Additionally, SNAPC1 facilitates the recruitment of TBP and BRF2 to the TATA box of the U6 snRNA gene.
Description
Recombinant Human SNAPC1, produced in E. coli, is an unglycosylated polypeptide chain consisting of 391 amino acids (residues 1-368). It has a molecular weight of 45.4 kDa. The protein is expressed with an N-terminal His-tag of 23 amino acids and purified using proprietary chromatographic methods.
Physical Appearance
Clear, sterile solution after filtration.
Formulation
The SNAPC1 protein solution is provided at a concentration of 1 mg/ml and contains 20 mM Tris-HCl (pH 8.0) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it should be kept frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity exceeds 80.0%, as determined by SDS-PAGE analysis.
Synonyms
PTFgamma, SNAP43, snRNA-activating protein complex subunit 1, SNAPc subunit 1, Proximal sequence element-binding transcription factor subunit gamma, PSE-binding factor subunit gamma, PTF subunit gamma, Small nuclear RNA-activating complex polypeptide 1, SNAPc 43 kDa subunit, SNAPC1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGTPPGL QTDCEALLSR FQETDSVRFE DFTELWRNMK FGTIFCGRMR NLEKNMFTKE ALALAWRYFL PPYTFQIRVG ALYLLYGLYN TQLCQPKQKI RVALKDWDEV LKFQQDLVNA QHFDAAYIFR KLRLDRAFHF TAMPKLLSYR MKKKIHRAEV TEEFKDPSDR VMKLITSDVL EEMLNVHDHY QNMKHVISVD KSKPDKALSL IKDDFFDNIK NIVLEHQQWH KDRKNPSLKS KTNDGEEKME GNSQETERCE RAESLAKIKS KAFSVVIQAS KSRRHRQVKL DSSDSDSASG QGQVKATRKK EKKERLKPAG RKMSLRNKGN VQNIHKEDKP LSLSMPVITE EEENESLSGT EFTASKKRRK H.

Product Science Overview

Introduction

The Small Nuclear RNA Activating Complex (SNAPc) is a crucial basal transcription factor involved in the transcription of small nuclear RNA (snRNA) genes by both RNA polymerase II and III. One of the key components of this complex is the Small Nuclear RNA Activating Complex, Polypeptide 1 (SNAPC1), also known as SNAP43 or PTFgamma .

Structure and Function

SNAPC1 is a protein-coding gene that encodes a 43 kDa subunit of the SNAPc complex. This complex is essential for the transcription of snRNA genes, which play a critical role in the splicing of pre-mRNA. The SNAPc complex binds specifically to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to snRNA genes .

The primary function of SNAPC1 within the SNAPc complex is to recruit transcription factors such as TBP (TATA-binding protein) and BRF2 to the U6 snRNA TATA box, facilitating the initiation of transcription . This recruitment is vital for the proper transcription of snRNA genes by RNA polymerase II and III.

Biological Significance

SNAPC1 is located in the nucleolus and nucleoplasm of cells, where it participates in the transcription of snRNA genes . The transcription of these genes is crucial for the production of snRNAs, which are integral components of the spliceosome. The spliceosome is responsible for the removal of introns from pre-mRNA, a critical step in the maturation of messenger RNA (mRNA).

Clinical Relevance

Mutations or dysregulation of SNAPC1 and other components of the SNAPc complex can potentially lead to defects in snRNA transcription and splicing, which may contribute to various genetic disorders and diseases. Understanding the function and regulation of SNAPC1 is therefore important for elucidating the molecular mechanisms underlying these conditions.

Research and Applications

Recombinant human SNAPC1 is used in various research applications to study the transcriptional regulation of snRNA genes and the assembly of the SNAPc complex. By using recombinant proteins, researchers can investigate the specific interactions and functions of SNAPC1 in a controlled environment, providing insights into its role in gene expression and potential therapeutic targets.

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