SMCP Human

Sperm Mitochondria-Associated Cysteine-Rich Protein Human Recombinant
Cat. No.
BT17379
Source
Escherichia Coli.
Synonyms
Sperm Mitochondria-Associated Cysteine-Rich Protein, MCSP, MCS, Mitochondrial Capsule Selenoprotein, HSMCSGEN1, Sperm Mitochondrial-Associated Cysteine-Rich Protein.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SMCP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 139 amino acids (1-116 a.a) and having a molecular mass of 15.2kDa (Molecular size on SDS-PAGE will appear higher). SMCP is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The SMCP gene encodes a protein found in the capsule associated with the outer membranes of mitochondria. This protein is believed to play a role in structuring and stabilizing the helical arrangement of the mitochondrial sheath within sperm cells. Sperm mitochondria exhibit distinct morphology and subcellular positioning compared to those found in somatic cells. They are characterized by an elongated, flattened shape and are helically coiled around the midpiece of the sperm flagellum. SMCP has been linked to certain medical conditions, including middle ear disease and hard palate cancer.
Description
Recombinant human SMCP, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 139 amino acids (with positions 1-116 representing the amino acid sequence) and possessing a molecular mass of 15.2 kDa. On SDS-PAGE, the apparent molecular size will appear larger. A 23 amino acid His-tag is fused to the N-terminus of the SMCP protein. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
The product is provided as a sterile, clear solution.
Formulation
The SMCP protein solution (0.5 mg/ml) is supplied in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 20% glycerol, 1 mM EDTA, and 0.1 mM PMSF.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the product is determined to be greater than 85.0% using SDS-PAGE analysis.
Synonyms
Sperm Mitochondria-Associated Cysteine-Rich Protein, MCSP, MCS, Mitochondrial Capsule Selenoprotein, HSMCSGEN1, Sperm Mitochondrial-Associated Cysteine-Rich Protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMCDQTKH SKCCPAKGNQ CCPPQQNQCC QSKGNQCCPP KQNQCCQPKG SQCCPPKHNH CCQPKPPCCI QARCCGLETK PEVSPLNMES EPNSPQTQDK GCQTQQQPHS PQNESRPSK.

Product Science Overview

Introduction

Sperm Mitochondria-Associated Cysteine-Rich Protein (SMCP), also known as mitochondrial capsule selenoprotein (MCS) or mitochondrial capsule protein (MCP), is a crucial protein localized in the mitochondrial capsule of sperm cells. This protein plays a significant role in the organization and stabilization of the helical structure of the sperm’s mitochondrial sheath .

Function and Importance

SMCP is involved in enhancing sperm motility, which is essential for successful fertilization. The protein’s absence has been linked to male infertility due to reduced sperm motility and the inability to penetrate the oocyte zona pellucida . The protein is rapidly evolving and is rich in cysteine and proline, contributing to its structural properties .

Localization and Structure

The protein encoded by the SMCP gene localizes to the capsule associated with the mitochondrial outer membranes. It is thought to function in the organization and stabilization of the helical structure of the sperm’s mitochondrial sheath . This unique localization and structure differentiate sperm mitochondria from those of somatic cells, which are typically elongated, flattened, and arranged circumferentially to form a helical coiled sheath in the midpiece of the sperm flagellum .

Autoantigen Properties

SMCP has been identified as an autoantigen in various studies. Autoimmunity to sperm can be induced by immunization with isologous or autologous spermatozoa, resulting in autoimmune orchitis and aspermatogenesis of the testis . The protein is recognized by antibodies generated through isologous immunization with sperm, demonstrating its role as a sperm autoantigen .

Genetic Information

The SMCP gene is a protein-coding gene associated with diseases such as Meckel Syndrome, Type 6, and male infertility . The gene is located on chromosome 1 and has several aliases, including MCSP and HSMCSGEN1 .

Research and Applications

Research on SMCP has provided insights into its role in sperm motility and fertility. The recombinant form of SMCP (human recombinant) is used in various studies to understand its biochemical characteristics and potential therapeutic applications. The protein’s role in enhancing sperm motility makes it a potential target for treating male infertility.

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