SMARCA4 Human

SMARCA4 Human Recombinant

Cat. No.

BT17289

Source

Escherichia Coli.

Synonyms

SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 4, Mitotic Growth And Transcription Activator, ATP-Dependent Helicase SMARCA4, BRG1, Global Transcription Activator Homologous Sequence Sucrose Nonfermenting-Like 4, BRG1-Associated Factor 190A, Protein Brahma Homolog 1, BRM/SWI2-Related Gene 1, Homeotic Gene Regulator, Brahma Protein-Like 1 Nuclear Protein GRB1, Protein BRG-1, SNF2-Like 4, SNF2-Beta, BAF190A, SNF2L4, MRD16, RTPS2, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 4, Transcription Activator BRG1, EC 3.6.4.-, EC 3.6.1, BAF190, SNF2LB, HSNF2b, SNF2B, SNF2, SWI2, Transcription activator BRG1.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

SMARCA4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 211 amino acids (362-549aa) and having a molecular mass of 25kDa.

SMARCA4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

SMARCA4, a member of the SWI/SNF protein family, shares similarities with the Drosophila brahma protein. These proteins, characterized by their helicase and ATPase activities, are believed to play a role in transcriptional regulation by influencing the chromatin structure surrounding specific genes. As a component of the ATP-dependent chromatin remodeling complex SNF/SWI, SMARCA4 is crucial for activating genes typically repressed by chromatin. Notably, SMARCA4 exhibits binding affinity for BRCA1 and participates in regulating the expression of CD44, a protein associated with tumor development. Mutations in SMARCA4 are implicated in rhabdoid tumor predisposition syndrome type 2.

Description

Recombinantly produced in E. coli, SMARCA4 Human Recombinant is a single, non-glycosylated polypeptide chain comprising 211 amino acids (362-549aa) with a molecular weight of 25kDa. The protein is engineered with a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic methods.

Physical Appearance

A clear solution that has been sterilized by filtration.

Formulation

The SMARCA4 protein solution is provided at a concentration of 1mg/ml and contains 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA, and 10% glycerol.

Stability

For optimal storage, the product should be refrigerated at 4°C if the entire vial will be used within 2-4 weeks. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. It's essential to avoid repeated cycles of freezing and thawing.

Purity

SDS-PAGE analysis indicates a purity greater than 80.0%.

Synonyms

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSDPVEILQ EREYRLQARI AHRIQELENL PGSLAGDLRT KATIELKALR LLNFQRQLRQ EVVVCMRRDT ALETALNAKA YKRSKRQSLR EARITEKLEK QQKIEQERKR RQKHQEYLNS ILQHAKDFKE YHRSVTGKIQ KLTKAVATYH ANTEREQKKE NERIEKERMR RLMAEDEEGY RKLIDQKKDK R.

Product Science Overview

Introduction

SMARCA4, also known as BRG1 (Brahma-related gene 1), is a core ATPase subunit of the SWI/SNF (SWItch/Sucrose Non-Fermentable) chromatin remodeling complex. This complex is evolutionarily conserved and plays a crucial role in regulating gene transcription, DNA repair, differentiation, and development by mobilizing nucleosomes.

Structure and Function

SMARCA4 is an ATP-dependent chromatin remodeling enzyme that modulates nucleosome positioning and structure. It is essential for the regulation of transcription through the control of chromatin structure. The SWI/SNF complex, which includes SMARCA4, facilitates the access of transcription factors to DNA by altering the chromatin landscape.

Role in Cancer

SMARCA4 functions as a tumor suppressor and is implicated in various human malignancies. Alterations in the SMARCA4 gene, such as truncating mutations, fusions, and homozygous deletions, lead to loss of function and are associated with several cancers, including lung, colon, bladder, and breast carcinomas. These alterations can result in either loss-of-function or gain-of-function effects, contributing to cancer development and progression.

Clinical Implications

SMARCA4 alterations are particularly significant in certain rare cancers, such as small cell carcinomas of the ovary hypercalcemic type (SCCOHT) and SMARCA4-deficient thoracic and uterine sarcomas. Germline variants in the SMARCA4 gene are linked to hereditary conditions like rhabdoid tumor predisposition syndrome-2 (RTPS2) and Coffin-Siris syndrome. These conditions are characterized by aggressive tumors and developmental delays, respectively.

Therapeutic Potential

Research into therapeutic strategies targeting SMARCA4-altered cancers is ongoing. Immune checkpoint blockade has shown promising responses in SCCOHT. Additionally, inhibitors targeting BET, EZH2, HDAC, CDK4/6, and FGFR, as well as agents inducing synthetic lethality via DNA damage repair impairment, are being explored.

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SMARCA4 Human

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